Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis
| Autor: | Ryoji Maekura, Haruka Kobayashi, Masato Katahira, Ichiro Nakagawa, Tsukasa Mashima, Yuriko Ozeki, Yukihiro Kaneko, Yukiko Ohara, Akihito Nishiyama, Sohkichi Matsumoto, Yutaka Yoshida, Yoshie Fujiwara, Saburo Yamamoto, Yasuo Tsunaka, Yoshitaka Tateishi, Kengo Kitadokoro, Fumio Arisaka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Bacterial Diseases
0301 basic medicine Male Models Molecular lcsh:Medicine Protein Structure Prediction Plant Science Biochemistry Plant Roots Protein Structure Secondary Medicine and Health Sciences Macromolecular Structure Analysis Medicine lcsh:Science education.field_of_study Multidisciplinary biology Plant Anatomy Circular Dichroism General Medicine Middle Aged Recombinant Proteins Actinobacteria DNA-Binding Proteins Infectious Diseases Tuberculosis Diagnosis and Management Female medicine.symptom Antibody General Agricultural and Biological Sciences Research Article Protein Binding Adult Protein Structure Tuberculosis Science 030106 microbiology Population Asymptomatic General Biochemistry Genetics and Molecular Biology Mycobacterium tuberculosis 03 medical and health sciences Young Adult Antigen Tuberculosis diagnosis Bacterial Proteins Diagnostic Medicine Humans education Molecular Biology Aged Binding Sites Bacteria Biology and life sciences business.industry lcsh:R Organisms Proteins Correction Tropical Diseases biology.organism_classification medicine.disease Virology Protein tertiary structure Root Structure Case-Control Studies Immunoglobulin G biology.protein lcsh:Q Protein Multimerization business Mycobacterium Tuberculosis |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 10, p e0204160 (2018) PLoS ONE, Vol 16, Iss 8, p e0256946 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Tuberculosis causes the highest mortality among all single infections. Asymptomatic tuberculosis, afflicting one third of the global human population, is the major source as 5–10% of asymptomatic cases develop active tuberculosis during their lifetime. Thus it is one of important issues to develop diagnostic tools for accurately detecting asymptomatic infection. Mycobacterial DNA-binding protein 1 (MDP1) is a major protein in persistent Mycobacterium tuberculosis and has potential for diagnostic use in detecting asymptomatic infection. However, a previous ELISA-based study revealed a specificity problem; IgGs against MDP1 were detected in both M. tuberculosis-infected and uninfected individuals. Although the tertiary structures of an antigen are known to influence antibody recognition, the MDP1 structural details have not yet been investigated. The N-terminal half of MDP1, homologous to bacterial histone-like protein HU, is predicted to be responsible for DNA-binding, while the C-terminal half is assumed as totally intrinsically disordered regions. To clarify the relationship between the MDP1 tertiary structure and IgG recognition, we refined the purification method, which allow us to obtain a recombinant protein with the predicted structure. Furthermore, we showed that an IgG-ELISA using MDP1 purified by our refined method is indeed useful in the detection of asymptomatic tuberculosis. |
| Databáze: | OpenAIRE |
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