β1- and αv-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments

Autor: Reinhard Fässler, Zhiqi Sun, Julien Polleux, Michaela-Rosemarie Hermann, Timothée Vignaud, Caroline C. Friedel, Manuel Théry, Herbert B. Schiller, Sara Zanivan, Matthias Mann, Aurelia Raducanu, Kay-E. Gottschalk
Přispěvatelé: Department of Molecular Medicine [Martinsreid], Max Planck Institute of Biochemistry (MPIB), Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Institut de Biosciences et de Biotechnologies de Grenoble (ex-IRTSV) (BIG), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Grenoble Alpes (UGA), Department of Proteomics and Signal Transduction, Institute for Informatics, Ludwig-Maximilians-Universität München (LMU), Institute of Experimental Physics, Universität Ulm - Ulm University [Ulm, Allemagne], Max-Planck-Institut für Biochemie = Max Planck Institute of Biochemistry (MPIB), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), ERC, DFG, Max Planck Society, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Rok vydání: 2012
Předmět:
MESH: Antigens
CD29
MESH: Extracellular Matrix Proteins
Male
[SDV]Life Sciences [q-bio]
fibroblast
MESH: Cellular Microenvironment
Extracellular matrix
Mice
0302 clinical medicine
Cell-matrix adhesion
Cell Movement
MESH: Fibronectins
MESH: RNA
Small Interfering
Myosin
MESH: Guanine Nucleotide Exchange Factors
Guanine Nucleotide Exchange Factors
MESH: Animals
RNA
Small Interfering
MESH: Cell Movement
0303 health sciences
Extracellular Matrix Proteins
rho-Associated Kinases
MESH: Integrin alphaV
biology
Integrin beta1
Cell biology
Extracellular Matrix
Cellular Microenvironment
Mammalia
RNA Interference
MESH: rho-Associated Kinases
Integrin alpha5beta1
Protein Binding
MESH: rhoA GTP-Binding Protein
MESH: Myosin Type II
integrin
MESH: Mice
Transgenic
MESH: RNA Interference
Integrin
Formins
MESH: Carrier Proteins
Mice
Transgenic
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Extracellular Matrix
Protein Serine-Threonine Kinases
adhesion site
MESH: Protein-Serine-Threonine Kinases
MESH: Cell Adhesion
Cell Line
Focal adhesion
03 medical and health sciences
Proto-Oncogene Proteins
Cell Adhesion
MESH: Protein Binding
Animals
Cell adhesion
MESH: Mice
030304 developmental biology
Myosin Type II
Focal Adhesions
MESH: Integrin alpha5beta1
Adhesome
traction forces
MESH: Focal Adhesions
Cell Biology
Fibroblasts
Integrin alphaV
MESH: Male
MESH: Cell Line
Fibronectins
MESH: Proto-Oncogene Proteins
Fibronectin
rigidity sensing
MESH: Fibroblasts
biology.protein
Carrier Proteins
rhoA GTP-Binding Protein
030217 neurology & neurosurgery
Rho Guanine Nucleotide Exchange Factors
Zdroj: Nature Cell Biology
Nature Cell Biology, Nature Publishing Group, 2013, 15 (6), pp.625-36. ⟨10.1038/ncb2747⟩
Nature Cell Biology, 2013, 15 (6), pp.625-36. ⟨10.1038/ncb2747⟩
ISSN: 1476-4679
1465-7392
Popis: Supplementary information; International audience; How different integrins that bind to the same type of extracellular matrix protein mediate specific functions is unclear. We report the functional analysis of β1- and αv-class integrins expressed in pan-integrin-null fibroblasts seeded on fibronectin. Reconstitution with β1-class integrins promotes myosin-II-independent formation of small peripheral adhesions and cell protrusions, whereas expression of αv-class integrins induces the formation of large focal adhesions. Co-expression of both integrin classes leads to full myosin activation and traction-force development on stiff fibronectin-coated substrates, with αv-class integrins accumulating in adhesion areas exposed to high traction forces. Quantitative proteomics linked αv-class integrins to a GEF-H1-RhoA pathway coupled to the formin mDia1 but not myosin II, and α5β1 integrins to a RhoA-Rock-myosin II pathway. Our study assigns specific functions to distinct fibronectin-binding integrins, demonstrating that α5β1integrins accomplish force generation, whereas αv-class integrins mediate the structural adaptations to forces, which cooperatively enable cells to sense the rigidity of fibronectin-based microenvironments.
Databáze: OpenAIRE
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