| Autor: |
Sudwarts, Ari, Ramesha, Supriya, Gao, Tianwen, Ponnusamy, Moorthi, Wang, Shuai, Hansen, Mitchell, Kozlova, Alena, Bitarafan, Sara, Kumar, Prateek, Beaulieu-Abdelahad, David, Zhang, Xiaolin, Collier, Lisa, Szekeres, Charles, Wood, Levi B., Duan, Jubao, Thinakaran, Gopal, Rangaraju, Srikant |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.19728391.v1 |
| Popis: |
Additional file 9: Fig. S9. Generation of BV2 KO microglia lacking BIN1 expression by CRISPR/Cas9 gene editing. (A) Lentiviral constructs expressing a sgRNA targeting a region within the Bin1 invariant exon 3 (KO) or a non-target sgRNA were used to generate stably transduced pools of BV2 KO and control (WT) cells. Two independent pools of WT and KO were further characterized. Sequencing (using a reverse primer) across the target sequence of the two KO pools as well as the sequences of individual cloned inserts from the PCR products are aligned to Bin1 exon 3 sequence. Numbering is based on the RefSeq NM_009668.2. (B) WT control and Bin1 KO pools retain similar morphology. (C) Immunoblot analysis demonstrates that stable Bin1 KO BV2 pools do not express BIN1 protein under basal conditions or following LPS stimulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
