Analysis of SOX2-Regulated Transcriptome in Glioma Stem Cells

Autor: Acanda de la Rocha, Arlet M., López-Bertoni, Hernando, Guruceaga, Elizabeth, González-Huarriz, Marisol, Martínez-Vélez, Naiara, Xipell, Enric, Fueyo, Juan, Gomez-Manzano, Candelaria, Alonso, Marta M.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
endocrine system
Microarrays
Library science
lcsh:Medicine
Biology
Biochemistry
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Extraction techniques
Medicine and Health Sciences
Genetics
media_common.cataloged_instance
Blastomas
Small interfering RNAs
European union
lcsh:Science
Non-coding RNA
Neurological Tumors
media_common
Multidisciplinary
Biology and life sciences
Gene Ontologies
lcsh:R
fungi
Cancers and Neoplasms
Computational Biology
General Medicine
Genomics
Genome Analysis
RNA extraction
Gene regulation
Nucleic acids
Research and analysis methods
030104 developmental biology
Bioassays and Physiological Analysis
Oncology
Neurology
Cancer research
Long non-coding RNAs
RNA
Christian ministry
lcsh:Q
Gene expression
General Agricultural and Biological Sciences
Transcriptome Analysis
Glioblastoma Multiforme
Research Article
Zdroj: PLoS ONE
Dadun. Depósito Académico Digital de la Universidad de Navarra
instname
PLOS ONE
PLoS ONE, Vol 11, Iss 9, p e0163155 (2016)
ISSN: 1932-6203
Popis: Introduction Glioblastoma is the most malignant brain tumor in adults and is associated with poor sur- vival despite multimodal treatments. Glioma stem-like cells (GSCs) are cells functionally defined by their self-renewal potential and the ability to reconstitute the original tumor upon orthotopic implantation. They have been postulated to be the culprit of glioma chemo- and radio-resistance ultimately leading to relapse. Understanding the molecular circuits govern- ing the GSC compartment is essential. SOX2, a critical transcription regulator of embryonic and neural stem cell function, is deregulated in GSCs however; the precise molecular path- ways regulated by this gene in GSCs remain poorly understood. Results We performed a genome-wide analysis of SOX2-regulated transcripts in GSCs, using a microarray. We identified a total of 2048 differentially expressed coding transcripts and 261 non-coding transcripts. Cell adhesion and cell-cell signaling are among the most enriched terms using Gene Ontology (GO) classification. The pathways altered after SOX2 down- modulation includes multiple cellular processes such as amino-acid metabolism and inter- cellular signaling cascades. We also defined and classified the set of non-coding transcripts differentially expressed regulated by SOX2 in GSCs, and validated two of them. Conclusions We present a comprehensive analysis of the transcriptome controlled by SOX2 in GSCs, gaining insights in the understanding of the potential roles of SOX2 in glioblastoma.
Databáze: OpenAIRE
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