RNAi-mediated silencing of vitellogenin gene function turns honeybee (Apis mellifera) workers into extremely precocious foragers
| Autor: | Zilá Luz Paulino Simões, Klaus Hartfelder, Adriana Mendes do Nascimento, David S. Marco Antonio, Karina Rosa Guidugli-Lazzarini |
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| Rok vydání: | 2008 |
| Předmět: |
food.ingredient
Zoology Polymerase Chain Reaction Vitellogenins Vitellogenin food RNA interference Yolk Abdomen Animals Gene silencing Gene Ecology Evolution Behavior and Systematics DNA Primers Analysis of Variance Life Cycle Stages Behavior Animal biology Ecology Feeding Behavior General Medicine Bees Juvenile Hormones Worker bee Titer Gene Expression Regulation Juvenile hormone biology.protein RNA Female RNA Interference |
| Zdroj: | Naturwissenschaften. 95:953-961 |
| ISSN: | 1432-1904 0028-1042 |
| DOI: | 10.1007/s00114-008-0413-9 |
| Popis: | The switch from within-hive activities to foraging behavior is a major transition in the life cycle of a honeybee (Apis mellifera) worker. A prominent regulatory role in this switch has long been attributed to juvenile hormone (JH), but recent evidence also points to the yolk precursor protein vitellogenin as a major player in behavioral development. In the present study, we injected vitellogenin double-stranded RNA (dsVg) into newly emerged worker bees of Africanized genetic origin and introduced them together with controls into observation hives to record flight behavior. RNA interference-mediated silencing of vitellogenin gene function shifted the onset of long-duration flights (>10 min) to earlier in life (by 3–4 days) when compared with sham and untreated control bees. In fact, dsVg bees were observed conducting such flights extremely precociously, when only 3 days old. Short-duration flights ( |
| Databáze: | OpenAIRE |
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