Characterization of a New Human Cell Line (CH-3573) Derived from a Grade II Chondrosarcoma with Matrix Production
| Autor: | Danielle de Jong, José Antonio López-Guerrero, Silvia Calabuig-Fariñas, Amando Peydró, Antonio Pellín, Rosario Gil Benso, Lara Navarro, Karoly Szuhai, Isidro Machado, Antonio Llombart-Bosch, Teresa San Miguel |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Karyotype Transplantation Heterologous Chondrosarcoma Mice Nude Bone Neoplasms Biology Pathology and Forensic Medicine Mice Grade II In vivo Cell Line Tumor medicine Animals Humans Bone tumor In Situ Hybridization Fluorescence Matrix General Medicine medicine.disease Immunohistochemistry Primary tumor In vitro Neoplasm Proteins Oncology Cell culture Tumor progression Grade II Chondrosarcoma Neoplasm Grading Cell line |
| Zdroj: | Pathology and Oncology Research, 18(4), 793-802 |
| ISSN: | 1532-2807 1219-4956 |
| DOI: | 10.1007/s12253-012-9505-0 |
| Popis: | Chondrosarcomas are malignant cartilage-forming tumors that represent the third most common malignant solid tumor of bone. In patients with grades II and III, local recurrence, increasing tumor size and dedifferentiation have been associated with lower survival rates. These biologically poorly-understood neoplasms vary considerably in clinical presentation and biological behavior. Cytogenetic studies have shown that heterogeneity is related to karyotypic complexity; moreover, alterations in the 9p21 locus and TP53 gene are related to disease progression. Despite the relatively high frequency of chondrosarcoma only a limited number of cell lines exist in the scientific community, limiting the possibility to study hypothesis-derived research or primary drug interaction necessary for pre-clinical studies. We report a chondrosarcoma cell line, CH-3573, derived from a primary tumor that may serve as a useful tool for both in vitro and in vivo models to study the molecular pathogenesis. In addition, xenograft passages in nude mice were studied to characterize the genetic stability over the course of tumor progression. In contrary to other reported cell lines, an important feature of our established cell line was the retained matrix production, a characteristic feature of a conventional grade II chondrosarcoma. The cell line (CH-3573) was characterized by pathological, immunohistochemical and molecular genetic methods. |
| Databáze: | OpenAIRE |
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