NAD-sensitive hydrogel for the release of macromolecules
| Autor: | Adrian Bowyer, Robert Eisenthal, John Hubble, M Tang, R Zhang |
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| Rok vydání: | 2004 |
| Předmět: |
Bioengineering
macromolecular substances Applied Microbiology and Biotechnology Diffusion Hemoglobins chemistry.chemical_compound Drug Delivery Systems Coated Materials Biocompatible Materials Testing Binding site Chromatography biology Ligand Cytochrome c technology industry and agriculture Cytochromes c Hydrogels NAD Dextran chemistry Covalent bond Delayed-Action Preparations Multiprotein Complexes Self-healing hydrogels Biophysics biology.protein NAD+ kinase Pharmaceutical Vehicles Lysozyme Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 87:791-796 |
| ISSN: | 1097-0290 0006-3592 |
| Popis: | A hydrogel membrane containing immobilized ligands and receptors was synthesized and investigated for the controlled diffusion of test proteins (cytochrome C and hemoglobin). Both Cibacron blue (ligand) and lysozyme (receptor) were covalently linked to dextran molecules that were subsequently crosslinked to form a gel. The resulting stable hydrogels contained both covalent and affinity crosslinks such that their intrinsic porosities were sensitive to competitive displacers of the affinity interaction between lysozyme and Cibacron blue. Transport experiments in a twin chamber diffusion cell showed that as NAD was added to the donor side, the dissociation of the binding sites between the Cibacron blue and the lysozyme led to an increase in protein diffusion through the hydrogel. The results showed that addition of NAD caused a saturable concentration-dependent increase in the transport of both cytochrome C and hemoglobin. This effect was shown to be both specific and reversible. |
| Databáze: | OpenAIRE |
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