Flavobacterium panici sp. nov. isolated from the rhizosphere of the switchgrass Panicum virgatum
| Autor: | Jia Xu, Stefanie P. Glaeser, John A. McInroy, Peter Kämpfer, Hans-Jürgen Busse, Dominique Clermont, Alexis Criscuolo |
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| Přispěvatelé: | Justus-Liebig-Universität Gießen (JLU), Auburn University (AU), Veterinärmedizinische Universität Wien, Collection de l'Institut Pasteur (CIP), Institut Pasteur [Paris], Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MESH: Sequence Analysis
DNA [SDV]Life Sciences [q-bio] Microbiology MESH: Bacterial Typing Techniques Flavobacterium panici MESH: Nucleic Acid Hybridization 03 medical and health sciences taxonomy Glycolipid MESH: Base Composition Genotype Nucleotide MESH: Phylogeny MESH: Rhizosphere Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification MESH: Phospholipids 0303 health sciences Rhizosphere biology 030306 microbiology MESH: Panicum Fatty acid MESH: Flavobacterium General Medicine MESH: Vitamin K 2 16S ribosomal RNA biology.organism_classification MESH: DNA Bacterial MESH: Alabama MESH: Fatty Acids MESH: RNA Ribosomal 16S chemistry MESH: Soil Microbiology [SDE]Environmental Sciences Taxonomy (biology) Flavobacterium |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2020, 70 (11), pp.5824-5831. ⟨10.1099/ijsem.0.004482⟩ International Journal of Systematic and Evolutionary Microbiology, 2020, 70 (11), pp.5824-5831. ⟨10.1099/ijsem.0.004482⟩ |
| ISSN: | 1466-5026 1466-5034 |
| DOI: | 10.1099/ijsem.0.004482⟩ |
| Popis: | A Gram-staining-negative non endospore-forming strain, PXU-55T, was isolated from the rhizosphere of the switchgrass Panicum virgatum and studied in detail to determine its taxonomic position. The results of 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Flavobacterium . The isolate shared highest 16S rRNA gene sequence similarities with the type strains of Flavobacterium chungangense (98.78 %) and Flavobacterium chilense (98.64 %). The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH) values between the PXU-55T genome assembly and the ones of the most closely related type strains of species of the genus Flavobacterium were 87.3 and 31.9% ( Flavobacterium defluvii ), and 86.1 and 29.9% ( Flavobacterium johnsoniae ). Menaquinone MK-6 was the major respiratory quinone. As major polar lipids, phosphatidylethanolamine, an ornithine lipid and the unidentified polar lipids L2, L3 and L4 lacking a functional group were found. Moderate to minor amounts of another ornithine lipid, the unidentified lipid L1 and a glycolipid were present, as well. The major polyamine is sym-homospermidine. The fatty acid profiles contained major amounts of iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH) and various hydroxylated fatty acids in smaller amounts, among them iso C16:0 3-OH, C16:0 3-OH and C15:0 3-OH, which supported the classification of the isolate as a member of the genus Flavobacterium . Physiological and biochemical characterisation and ANI calculations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of the strain. For this reason, we propose that strain PXU-55T (=CIP 111646T=CCM 8914T) represents a novel species with the name Flavobacterium panici sp. nov. |
| Databáze: | OpenAIRE |
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