An open-source control system for in vivo fluorescence measurements from deep-brain structures
| Autor: | Anatol C. Kreitzer, Scott F. Owen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Optical fiber Computer science computer.software_genre Article law.invention Photometry (optics) Mice 03 medical and health sciences 0302 clinical medicine Software law In vivo fluorescence Animals Fluorometry Calcium Signaling Optical Fibers 030304 developmental biology 0303 health sciences Firmware business.industry General Neuroscience Optical Imaging Equipment Design Fluorescence Corpus Striatum Biophotonics Microcontroller 030104 developmental biology visual_art Control system Electronic component visual_art.visual_art_medium business computer 030217 neurology & neurosurgery Computer hardware |
| Zdroj: | Journal of Neuroscience Methods. 311:170-177 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/j.jneumeth.2018.10.022 |
| Popis: | BackgroundIntracranial photometry through chronically implanted optical fibers is a widely adopted technique for measuring signals from fluorescent probes in deep-brain structures. The recent proliferation of bright, photo-stable, and specific genetically-encoded fluorescent reporters for calcium and for other neuromodulators has greatly increased the utility and popularity of this technique.New MethodHere we describe an open-source, cost-effective, microcontroller-based solution for controlling optical components in an intracranial photometry system and processing the resulting signal.ResultsWe show proof-of-principle that this system supports high quality intracranial photometry recordings from dorsal striatum in freely moving mice. A single system supports simultaneous fluorescence measurements in two independent color channels, but multiple systems can be integrated together if additional fluorescence channels are required. This system is designed to work in combination with either commercially available or custom-built optical components. Parts can be purchased for less than one tenth the cost of commercially available alternatives and complete assembly takes less than one day for an inexperienced user.Comparison with Existing Method(s)Currently available hardware draws on a variety of commercial, custom-built, or hybrid elements for both optical and electronic components. Many of these hardware systems are either specialized and inflexible, or over-engineered and expensive.ConclusionsThis open-source system increases experimental flexibility while reducing cost relative to current commercially available components. All software and firmware are open-source and customizable, affording a degree of experimental flexibility that is not available in current commercial systems. |
| Databáze: | OpenAIRE |
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