| Autor: |
Ambuj Upadhyay, Nathan R Feltman, Adam Sychla, Anna Janzen, Siba R Das, Maciej Maselko, Michael Smanski |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
eLife. 11 |
| ISSN: |
2050-084X |
| Popis: |
Engineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated by introducing conditional female-lethality to EGI strains to generate a sex-sorting incompatible male system (SSIMS). Here, we demonstrate a proof of concept by combining tetracycline-controlled female lethality constructs with a pyramus-targeting EGI line in the model insect Drosophila melanogaster. We show that both functions (incompatibility and sex-sorting) are robustly maintained in the SSIMS line and that this approach is effective for population suppression in cage experiments. Further we show that SSIMS males remain competitive with wild-type males for reproduction with wild-type females, including at the level of sperm competition. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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