Tear and serum soluble leukocyte activation markers in conjunctival allergic diseases
| Autor: | Antonio G. Secchi, Franco Borghesan, Mario Plebani, Andrea Leonardi, Diego Faggian, Massimo DePaoli |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Allergy Adolescent Neutrophils Keratoconjunctivitis Eosinophil-Derived Neurotoxin Neutrophil Activation Conjunctivitis Bacterial Ribonucleases Leukocytes medicine Humans Conjunctivitis Allergic Peroxidase Bacterial Conjunctivitis Eosinophil cationic protein Bacterial disease business.industry Proteins Receptors Interleukin-2 Blood Proteins Eosinophil Granule Proteins Eosinophil medicine.disease eye diseases Ophthalmology medicine.anatomical_structure Tears Immunology Female sense organs Inflammation Mediators business Cell activation Vernal keratoconjunctivitis Biomarkers Histamine |
| Zdroj: | American Journal of Ophthalmology. 129:151-158 |
| ISSN: | 0002-9394 |
| Popis: | PURPOSE: To measure markers of leukocyte activation in patients with an exclusively ocular inflammatory or bacterial disease. METHODS: Neutrophil myeloperoxidase, eosinophil cationic protein, eosinophil neurotoxin, and soluble interleukin-2 receptor were measured in serum and tears of 17 patients with allergic vernal keratoconjunctivitis, seven with atopic keratoconjunctivitis, 11 with seasonal allergic conjunctivitis, seven with giant papillary conjunctivitis, 13 with rosacea blepharokeratoconjunctivitis, seven with bacterial conjunctivitis, and 13 normal subjects as controls. RESULTS: In serum of patients with vernal and atopic keratoconjunctivitis, levels of eosinophil cationic protein, eosinophil neurotoxin, and interleukin-2 receptor were significantly increased compared with control subjects but were not correlated with the severity of ocular symptoms. In tears of patients with vernal and atopic keratoconjunctivitis and seasonal allergic conjunctivitis, as well as in the nonallergic diseases, rosacea blepharokeratoconjunctivitis and bacterial conjunctivitis, levels of eosinophil cationic protein, neurotoxin, and interleukin-2 receptor were significantly increased compared with control subjects. The highest values of these markers were found in vernal keratoconjunctivitis samples. Neutrophil myeloperoxidase was significantly increased in vernal and atopic keratoconjunctivitis, rosacea blepharokeratoconjunctivitis, and bacterial conjunctivitis. In vernal keratoconjunctivitis, tear markers were correlated to the clinical score of the disease, but not with cytology. CONCLUSIONS: Tear histamine was measured in 10 allergic patients after allergen challenge. Although none of the above markers can be considered specific to a single disease, their measurement may still be useful for the quantification of local cell activation in ocular inflammatory diseases. |
| Databáze: | OpenAIRE |
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