Antioxidant and Cytoprotective Effect of Piper aduncum L. against Sodium Fluoride (NaF)-Induced Toxicity in Albino Mice
| Autor: | Ricardo Ángel Yuli-Posadas, Luz Chacaltana-Ramos, Juan Pedro Rojas-Armas, Cesar Franco-Quino, Bertha Pari-Olarte, Edwin Enciso-Roca, Luis Miguel Visitación Felix-Veliz, Johnny Aldo Tinco-Jayo, Oscar Herrera-Calderon |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Piper aduncum
Antioxidant antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment Toxicology medicine.disease_cause lcsh:Chemical technology 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sodium fluoride medicine lcsh:TP1-1185 Comet assay Chemical Health and Safety Traditional medicine biology cytoprotective biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry micronucleus test chemistry 030220 oncology & carcinogenesis Toxicity Micronucleus test Micronucleus Genotoxicity |
| Zdroj: | Toxics, Vol 7, Iss 2, p 28 (2019) Toxics Volume 7 Issue 2 |
| ISSN: | 2305-6304 |
| Popis: | Piper aduncum, commonly known as matico, is a plant that grows in the mountainous and coastal regions of Peru, and is studied for its antimicrobial properties and various ethnopharmacological uses. The main objective of this study was to determine the cytoprotective and antioxidant effects of the methanolic extract of Piper aduncum leaves in Mus musculus previously administered with sodium fluoride (NaF) using the Micronucleus test and the Comet assay. The extract was administrated orally in four different concentrations: 150, 300, 600, and 1200 mg/Kg for ten days. At the 11th day, a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was performed with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according to OECD guidelines 474 and 489, respectively. The statistical analysis was performed by median analysis with ANOVA. Significant differences were found in Micronucleus frequency between the highest concentrations of Piper aduncum and NaF. The Comet assay showed significant reduction of NaF-induced damage on erythrocytes depending on the different concentrations of the extract which were evaluated in this study. It is concluded that the methanolic extract of P. aduncum leaves has cytoprotective and antioxidant activity against sodium fluoride. |
| Databáze: | OpenAIRE |
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