Hydrodynamic trapping measures the interaction between membrane-associated molecules

Autor: Ana Filipa L.O.M. Santos, Jana Hladilkova, Peter Jönsson, Victoria Junghans, Simon J. Davis, Mikael Lund
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-018-30285-0
Popis: How membrane proteins distribute and behave on the surface of cells is determined by the molecules’ interaction potential. However, measuring this potential, and how it varies with protein-to-protein distance, has been challenging. We here present how a method we call hydrodynamic trapping can achieve this. Our method uses the focused liquid flow from a micropipette to locally accumulate molecules protruding from a lipid membrane. The interaction potential, as well as information about the dimensions of the studied molecule, are obtained by relating the degree of accumulation to the strength of the trap. We have used this to study four representative proteins, with different height-to-width ratios and protein properties; from the globular streptavidin, to the rod-like immune cell proteins CD2, CD4 and CD45. The obtained data illustrates how protein shape, glycosylation and flexibility influence the behaviour of membrane proteins as well as underline the general applicability of the method.
Databáze: OpenAIRE
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