Expression of retrovirally transduced genes in primary cultures of adult rat hepatocytes
| Autor: | Hyam L. Leffert, James G. Respess, Jane C. Moores, Theodore Friedmann, Jiing-Kuan Yee, Jon A. Wolff, H. Skelly |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Hypoxanthine Phosphoribosyltransferase
Genes Viral Transcription Genetic Genetic Vectors Biology chemistry.chemical_compound Transduction Genetic Gene expression medicine Animals Humans Cells Cultured Serum Albumin Hypoxanthine Multidisciplinary Genetic transfer Neomycin Provirus Cell cycle Molecular biology Rats Leukemia Virus Murine medicine.anatomical_structure Genes Liver chemistry Cell culture Hepatocyte Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 84:3344-3348 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.84.10.3344 |
| Popis: | Differentiated primary rat hepatocyte cultures have been infected with retroviral vectors expressing human hypoxanthine/guanine phosphoribosyltransferase or the transposon Tn5 neomycin-resistance gene. Expression of the markers was detected only after infection of the cells during a short period of cell replication and transient dedifferentiation from days 1 to 5 of culture. Provirus integrated during that period remains fully expressed during the entire subsequent stationary period of culture up to at least 25 days. Selection with the neomycin analogue G418 of cells infected with the neomycin vector led to the appearance of cells with hepatocyte morphology in which newly synthesized albumin was detectable by immunoprecipitation, indicating successful infection of hepatocytes. |
| Databáze: | OpenAIRE |
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