Neuroblastoma tumorigenesis is regulated through the Nm23-H1/h-Prune C-terminal interaction
| Autor: | Alexander Schramm, Marianeve Carotenuto, Emilia Pedone, Stefania Correale, Fabio Pastorino, Massimo Zollo, Sašo Džeroski, Natascia Marino, Luciano Pirone, Gianluigi Arrigoni, Flora Cimmino, Luigi Navas, Ivica Slavkov, Giuseppe Basso, Johannes H. Schulte, Benedetta Accordi, Simona Maria Monti, Valeria Di Dato, Roberto Fattorusso, Maria Nunzia Scoppettuolo, Bernard Ženko, Frank Westermann, Pasqualino De Antonellis, Michele Saviano, Angelika Eggert, Mirco Ponzoni, Elisabeth Bruder, Donatella Diana |
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| Přispěvatelé: | Carotenuto, M, Pedone, E, Diana, D, de Antonellis, P, Džeroski, S, Marino, N, Navas, Luigi, Di Dato, V, Scoppettuolo, Mn, Cimmino, F, Correale, S, Pirone, L, Monti, Sm, Bruder, E, Zenko, B, Slavkov, I, Pastorino, F, Ponzoni, M, Schulte, Jh, Schramm, A, Eggert, A, Westermann, F, Arrigoni, G, Accordi, B, Basso, G, Saviano, M, Fattorusso, R, Zollo, Massimo, Navas, L, Fattorusso, Roberto, Zollo, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular Candidate gene Magnetic Resonance Spectroscopy Medizin medicine.disease_cause Pathogenesis Mice Neuroblastoma 0302 clinical medicine Cell Movement Neoplasm Metastasis chemistry.chemical_classification 0303 health sciences Multidisciplinary NM23 Nucleoside Diphosphate Kinases Immunohistochemistry 3. Good health Amino acid Gene Expression Regulation Neoplastic Cell Transformation Neoplastic 030220 oncology & carcinogenesis Female Protein Binding Blotting Western Transplantation Heterologous Mice Nude Motility Biology TRIM22 Article 03 medical and health sciences Cell Line Tumor medicine Animals Humans Gene 030304 developmental biology Binding Sites medicine.disease Molecular biology Phosphoric Monoester Hydrolases Protein Structure Tertiary HEK293 Cells chemistry nervous system Mutation Carrier Proteins Peptides Carcinogenesis |
| Zdroj: | Scientific reports (Nature Publishing Group) 3 (2013). doi:10.1038/srep01351 info:cnr-pdr/source/autori:Carotenuto, M ; Pedone, E ; Diana, D ; de Antonellis, P ; Dzeroski, S ; Marino, N ; Navas, L ; Di Dato, V ; Scoppettuolo, MN ; Cimmino, F ; Correale, S ; Pirone, L ; Monti, SM ; Bruder, E ; Zenko, B ; Slavkov, I ; Pastorino, F ; Ponzoni, M ; Schulte, JH ; Schramm, A ; Eggert, A; Westermann, F ; Arrigoni, G ; Accordi, B ; Basso, G ; Saviano, M ; Fattorusso, R ; Zollo, M/titolo:Neuroblastoma tumorigenesis is regulated through the Nm23-H1%2Fh-Prune C-terminal interaction/doi:10.1038%2Fsrep01351/rivista:Scientific reports (Nature Publishing Group)/anno:2013/pagina_da:/pagina_a:/intervallo_pagine:/volume:3 Scientific Reports; Vol 3 Scientific Reports |
| Popis: | Nm23-H1 is one of the most interesting candidate genes for a relevant role in Neuroblastoma pathogenesis. H-Prune is the most characterized Nm23-H1 binding partner, and its overexpression has been shown in different human cancers. Our study focuses on the role of the Nm23-H1/h-Prune protein complex in Neuroblastoma. Using NMR spectroscopy, we performed a conformational analysis of the h-Prune C-terminal to identify the amino acids involved in the interaction with Nm23-H1. We developed a competitive permeable peptide (CPP) to impair the formation of the Nm23-H1/h-Prune complex and demonstrated that CPP causes impairment of cell motility, substantial impairment of tumor growth and metastases formation. Meta-analysis performed on three Neuroblastoma cohorts showed Nm23-H1 as the gene highly associated to Neuroblastoma aggressiveness. We also identified two other proteins (PTPRA and TRIM22) with expression levels significantly affected by CPP. These data suggest a new avenue for potential clinical application of CPP in Neuroblastoma treatment. |
| Databáze: | OpenAIRE |
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