Sensitive determination of Hg(II) based on a hybridization chain recycling amplification reaction and surface-enhanced Raman scattering on gold nanoparticles
| Autor: | Shaoping Lv, Yunxia Li, Yan Gong, Caifeng Ding, Ruiyuan Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
Streptavidin
Detection limit 010401 analytical chemistry Analytical chemistry Nanoparticle Nanoprobe Nanochemistry 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound symbols.namesake chemistry Colloidal gold symbols 0210 nano-technology Raman spectroscopy Raman scattering |
| Zdroj: | Microchimica Acta. 185 |
| ISSN: | 1436-5073 0026-3672 |
| DOI: | 10.1007/s00604-018-2907-2 |
| Popis: | A method was developed for the determination of mercuric ion Hg(II). It is based on hybridization chain reaction (HCR) and surface-enhanced Raman scattering (SERS). Raman signal DNA and streptavidin were self-assembled on gold nanoparticles as a novel signal nanoprobe (AuNP-sDNA). A thymine-mercury(II)-thymine structure was immobilized on magnetic beads (MBs). The HCR makes use of two hairpin probes that are initiated by the trigger DNA to form a stable nicked dsDNA structure (MB-TS-hDNAs). A large number of the binding sites is provided to connect the signal nanoprobe. The stable sandwich structure (MB-TS-hDNA/AuNP-sDNA) was isolated by applying a magnetic field and used in the amplification step. In this way, Hg(II) can be determined sensitively after multiple signal amplification. The SERS signal, measured at 1499 cm−1, increases linearly in the 0.1 pM to 10 nM Hg(II) concentration range, and the limit of detection is 0.08 pM (at an S/N ratio of 3). The method was applied to the detection of Hg(II) in spiked environment water samples, with recoveries ranging from 96 to 119%. |
| Databáze: | OpenAIRE |
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