Human brain in tissue culture. III. PML-SV40-induced transformation of brain cells and establishment of permanent lines
| Autor: | Zofia Wroblewska, Daniela Santoli, Hilary Koprowski, Donald H. Gilden, Anthony J. Girardi |
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| Rok vydání: | 1975 |
| Předmět: |
Pathology
medicine.medical_specialty Multiple Sclerosis Time Factors Central nervous system Growth Simian virus 40 Biology Creutzfeldt-Jakob Syndrome Virus Cell Line Tissue culture Cricetinae Methods medicine Animals Humans Amyotrophic lateral sclerosis Antigens Viral Cells Cultured General Neuroscience Multiple sclerosis Amyotrophic Lateral Sclerosis Leukoencephalopathy Progressive Multifocal Brain Human brain medicine.disease Transformation (genetics) Cell Transformation Neoplastic medicine.anatomical_structure Karyotyping Explant culture |
| Zdroj: | The Journal of Comparative Neurology. 161:317-328 |
| ISSN: | 1096-9861 0021-9967 |
| Popis: | Cells from explants and monolayer subcultures of adult human brain obtained from biopsies or autopsies of ten multiple sclerosis (MS) cases, one case each of Jakob-Creutzfeld (JC) and amyotrophic lateral sclerosis (ALS) and three cases with no central nervous system (CNS) involvement were transformed with PML-SV40 virus. Transformation was effected to establish permanent lines of these particular adult brain cells so that sufficient quantities would be available for other research projects. The procedure previously used to transform human fibroblasts (Koprowski et al., '62) was successfully applied to human brain cells. The success of transformation was dependent on the growth condition of the cultures at the time of infection. Events occurring after viral infection and during the pre-transformation and the post-transformation phases are described. |
| Databáze: | OpenAIRE |
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