In vivo glucose imaging in multiple model organisms with an engineered single-wavelength sensor
| Autor: | Soomin Kim, Jonathan S. Marvin, William C. Lemon, Haluk Lacin, Jamien Shea, Philipp J. Keller, Richard T. Lee, Loren L. Looger, Minoru Koyama, Jacob P. Keller |
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| Rok vydání: | 2021 |
| Předmět: |
Central Nervous System
0301 basic medicine medicine.medical_treatment Glucose uptake Central nervous system Models Biological General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine medicine Neuropil Animals Humans Zebrafish Chemistry Muscles Insulin Glucose transporter Proteins Biological Transport Metabolism Cell biology Glucose HEK293 Cells 030104 developmental biology medicine.anatomical_structure Larva Drosophila Neuron Genetic Engineering Neuroglia 030217 neurology & neurosurgery Astrocyte |
| Zdroj: | Cell Reports. 35:109284 |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2021.109284 |
| Popis: | Summary Glucose is arguably the most important molecule in metabolism, and its dysregulation underlies diabetes. We describe a family of single-wavelength genetically encoded glucose sensors with a high signal-to-noise ratio, fast kinetics, and affinities varying over four orders of magnitude (1 μM to 10 mM). The sensors allow mechanistic characterization of glucose transporters expressed in cultured cells with high spatial and temporal resolution. Imaging of neuron/glia co-cultures revealed ∼3-fold faster glucose changes in astrocytes. In larval Drosophila central nervous system explants, intracellular neuronal glucose fluxes suggested a rostro-caudal transport pathway in the ventral nerve cord neuropil. In zebrafish, expected glucose-related physiological sequelae of insulin and epinephrine treatments were directly visualized. Additionally, spontaneous muscle twitches induced glucose uptake in muscle, and sensory and pharmacological perturbations produced large changes in the brain. These sensors will enable rapid, high-resolution imaging of glucose influx, efflux, and metabolism in behaving animals. |
| Databáze: | OpenAIRE |
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