POSSIBLE REGULATION OF LDL-RECEPTOR BY NARINGENIN IN HEPG2 HEPATOMA CELL LINE
Autor: | Nora A. Bawazeer, Hani Choudary, Mustafa Zeyadi, Ashwag Albukhari, Mazin A. Zamzami, Wesam H. Abdulaal, Said S Moselhy, Bruce Middleton |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Naringenin medicine.medical_specialty Carcinoma Hepatocellular LDL-receptor – Naringenin- HepG2 Article Phosphatidylinositol 3-Kinases 03 medical and health sciences chemistry.chemical_compound Internal medicine Drug Discovery Gene expression medicine Humans Protein maturation Regulation of gene expression biology Liver Neoplasms food and beverages Promoter Hep G2 Cells Fatty acid synthase LDL-receptor - Naringenin- HepG2 030104 developmental biology Endocrinology Gene Expression Regulation Receptors LDL Complementary and alternative medicine chemistry Flavanones LDL receptor biology.protein lipids (amino acids peptides and proteins) Sterol regulatory element-binding protein 2 Sterol Regulatory Element Binding Protein 1 Sterol Regulatory Element Binding Protein 2 |
Zdroj: | African Journal of Traditional, Complementary and Alternative Medicines; Vol 14, No 1 (2017); 278-287 African Journal of Traditional, Complementary, and Alternative Medicines |
ISSN: | 2505-0044 0189-6016 |
Popis: | Background: High plasma concentration of low-density lipoprotein cholesterol (LDL-c) plays a significant role in the incidence of atherosclerosis and coronary heart diseases (CHD).Materials and Methods: The purpose of this study was to investigate the mechanism by which citrus flavonoids, naringenin regulate the LDL receptor (LDLr) gene in human liver using the human hepatoma cell line, HepG2 as a model.Results: Time-course transient transfection of HepG2 cells with luciferase reporter-gene constructs incorporating the promoters of SREBP-1a,-1c, -2 and LDLr, revealed that in lipoprotein-deficient medium (LPDM), only SREBP-1a promoter activity was increased significantly after 4h exposure to 200μM naringenin respectively. However, after 24h incubation with 200μM naringenin the gene expression activities of all the SREBP-1a, -1c, -2 and LDLr promoterconstructs were increased significantly. The effects of both 200μM naringenin on elevating LDLr mRNA are possibly due to regulation of gene transcription by SREBP-la and SREBP-2. However, the suppression effect of 200μM naringenin on hepatic SREBP-1c mRNA expression is likely associated with the reduction in mRNA expression of both acetyl-CoA carboxylase and fatty acid synthase in human hepatoma HepG2 cells. It was found that, 200μM naringenin was likely to stimulate LDLr gene expression via increase phosphorylation of PI3K and ERK1/2 which enhance the transcription factors SREBP-1a and SREBP-2 mRNA levels and increased their protein maturation in human hepatoma HepG2 cell.Conclusion: Diets supplemented with naringenin could effectively reduce mortality and morbidity from coronary heart diseases and as cardio-protective effects in humans.Keywords: LDL-receptor – Naringenin- HepG2 |
Databáze: | OpenAIRE |
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