Biofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes
| Autor: | Gizem Arık, Berna Akova-Budak, Merih Kıvanç, Sertaç Argun Kıvanç |
|---|---|
| Přispěvatelé: | Anadolu Üniversitesi, Eczacılık Fakültesi, Mikrobiyoloji Anabilim Dalı, Kıvanç, Merih |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.drug_class Antibiotics medicine.disease_cause Microbiology Agar plate 03 medical and health sciences 0302 clinical medicine Antibiotic resistance medicine Ocular Surface biology Staphylococcus Spp business.industry Biofilm Diabetes General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification biofilm Staphylococcus spp antibiotic resistance ocular surface Ciprofloxacin 030104 developmental biology Antibiotic Resistance 030221 ophthalmology & optometry Vancomycin business Staphylococcus Bacteria medicine.drug |
| Zdroj: | Malawi Medical Journal; Vol 30, No 4 (2018); 243-249 |
| ISSN: | 1995-7262 |
| DOI: | 10.4314/mmj.v30i4.6 |
| Popis: | WOS: 000456650500006 Introduction Bacterial biofilm is an exopolysaccharide matrix that is produced by bacteria while they adhere on abiotic or biotic surfaces. The bacteria living in this matrix are more resistant to antibiotics than planctonic bacteria. The biofilm formation property of the bacteria is determined by genes; and this is related to virulence of the microorganism. In ophthalmology, biofilms form especially on abiotic surfaces such as silicon tubes, contact lenses, intraocular lenses etc. Aim Our aim was to investigate genotypic and phenotypic structures of biofilms that are produced by Staphylococcus spp., which was obtained from the eyes of diabetic patients and determine the effect on antibiotic susceptibility. Methods The study group was comprised with 83 isolates from diabetic patients and 21 isolates from non-diabetic patients. Presumptive isolates were detected and confirmed by a microbial identification system VITEK II. Automated EcoRI Ribotyping was performed. Biofilm production was detected by Congo Red Agar Plate and Microtiter Plate Assay. Disc diffusion method was used for determination of antibiotic susceptibility of isolates. Results Out of the 83 isolates from diabetic patients, 25 were weakly (30%), 20 were moderately (24%), and 25 were strongly (30%) biofilm positive. Seven isolates of S. aureus, 11 isolates of S. epidermidis, 2 isolates of S. warneri, 3 isolates of S. hominis, and 2 isolates of S. lugdunensis were identified as strong biofilm producers. Out of the 83 Staphylococcus isolates, 37 were cefuroxime, 18 ciprofloxacin, 11 vancomycin, 12 gatifloxacin, and 18 moxifloxacin resistant. In total, 37 strains were resistant to three or more antibiotics. There was a statistically significant relation between biofilm formation and multidrug resistance (against three or more antibiotics,p Anadolu University of Research Project Fund [1306F164] This study was supported by "Anadolu University of Research Project Fund" (Project No: 1306F164) |
| Databáze: | OpenAIRE |
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