Experimental Pretreatment with Chlorogenic Acid Prevents Transient Ischemia-Induced Cognitive Decline and Neuronal Damage in the Hippocampus through Anti-Oxidative and Anti-Inflammatory Effects

Autor: Hye Jin Sim, Myoung Cheol Shin, Bora Kim, Dae Won Kim, Young-Myeong Kim, Joon Ha Park, Jun Hwi Cho, Tae-Kyeong Lee, Ji Hyeon Ahn, Jae-Chul Lee, Moo Ho Won, Sungwoo Ryoo, Il-Jun Kang, Soo Young Choi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
endocrine system
Antioxidant
medicine.medical_treatment
chlorogenic acid
hippocampal neurons
SOD2
Anti-Inflammatory Agents
Pharmaceutical Science
Pharmacology
medicine.disease_cause
Neuroprotection
Hippocampus
Article
Antioxidants
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Cognition
lcsh:Organic chemistry
Chlorogenic acid
Ischemia
Drug Discovery
medicine
Caffeic acid
Animals
Physical and Theoretical Chemistry
Cognitive decline
030304 developmental biology
Neurons
0303 health sciences
Aldehydes
Chemistry
Superoxide Dismutase
Organic Chemistry
Interleukin
anti-inflammation
Neuroprotective Agents
Chemistry (miscellaneous)
antioxidation
Molecular Medicine
Interleukin-2
neuroprotection
Interleukin-4
transient forebrain ischemia
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Molecules
Volume 25
Issue 16
Molecules, Vol 25, Iss 3578, p 3578 (2020)
ISSN: 1420-3049
Popis: Chlorogenic acid (CGA), an ester of caffeic acid and quinic acid, is among the phenolic acid compounds which can be naturally found in green coffee extract and tea. CGA has been studied since it displays significant pharmacological properties. The aim of this study was to investigate the effects of CGA on cognitive function and neuroprotection including its mechanisms in the hippocampus following transient forebrain ischemia in gerbils. Memory and learning following the ischemia was investigated by eight-arm radial maze and passive avoidance tests. Neuroprotection was examined by immunohistochemistry for neuronal nuclei-specific protein and Fluoro-Jade B histofluorescence staining. For mechanisms of the neuroprotection, alterations in copper, zinc-superoxide dismutase (SOD1), SOD2 as antioxidant enzymes, dihydroethidium and 4-hydroxy-2-nonenal as indicators for oxidative stress, and anti-inflammatory cytokines (interleukin (IL)-4 and IL-13) and pro-inflammatory cytokines (tumor necrosis factor &alpha
(TNF-&alpha
) and IL-2) were examined by Western blotting and/or immunohistochemistry. As a result, pretreatment with 30 mg/kg CGA attenuated cognitive impairment and displayed a neuroprotective effect against transient forebrain ischemia (TFI). In Western blotting, the expression levels of SOD2 and IL-4 were increased due to pretreatment with CGA and, furthermore, 4-HNE production and IL-4 expressions were inhibited by CGA pretreatment. Additionally, pretreated CGA enhanced antioxidant enzymes and anti-inflammatory cytokines and, in contrast, attenuated oxidative stress and pro-inflammatory cytokine expression. Based on these results, we suggest that CGA can be a useful neuroprotective material against ischemia-reperfusion injury due to its antioxidant and anti-inflammatory efficacies.
Databáze: OpenAIRE
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