Bacterial adherence and glycocalyx formation in osteomyelitis experimentally induced with Staphylococcus aureus
| Autor: | Tober-Meyer B, Costerton Jw, Mayberry-Carson Kj, J K Smith, Lambe Dw |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
Staphylococcus aureus Pathology medicine.medical_specialty Medullary cavity Immunology Biology medicine.disease_cause Staphylococcal infections Microbiology Catheterization Glycocalyx Bone Marrow medicine Animals Tibia Osteomyelitis Adhesiveness Staphylococcal Infections medicine.disease Ruthenium Red Disease Models Animal Catheter Infectious Diseases medicine.anatomical_structure Parasitology Rabbits Bone marrow Osteitis Research Article Sodium Morrhuate |
| Zdroj: | Infection and Immunity. 43:825-833 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.43.3.825-833.1984 |
| Popis: | A surgical procedure allowed the placement of a silicone rubber catheter in the marrow cavity of the tibia of a rabbit and also allowed the introduction of a sclerosing agent (sodium morrhuate) and cells of Staphylococcus aureus. Osteomyelitis developed in 60% of the animals so treated, and the infecting microorganism was recovered from the infected tibias of the animals that developed this disease. All blood cultures taken 24 h after the infection were negative for S. aureus. Radiological findings consisted of osteolytic changes, the occurrence of sequestration and periosteal reactions, and sclerosis in the infected bones. Sections of bone prepared for histological examination confirmed the diagnosis of osteomyelitis. Transmission and scanning electron microscopy of samples of bone marrow, bone chips, and the catheters taken from the infected tibiae revealed gram-positive cocci embedded in a very extensive matrix of ruthenium red-staining glycocalyx adhering to the bone and the implanted catheter. It is proposed that this extensive glycocalyx served a protective function for the bacteria and was important in bacterial adherence and thus played an important role in bacterial persistence and the development of osteomyelitis in these rabbits. |
| Databáze: | OpenAIRE |
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