Combined antibacterial and osteogenic in situ effects of a bifunctional titanium alloy with nanoscale hydroxyapatite coating

Autor: Wen-Hao Hu, Yong-Gang Zhou, Jiu-Zheng Deng, Song-Hua Xiao, Daixu Wei, Kai Xu, Jian-Jin Zhu, Hua-Wei Liu
Rok vydání: 2018
Předmět:
0301 basic medicine
In situ
Materials science
Surface Properties
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
chemistry.chemical_element
02 engineering and technology
Gram-Positive Bacteria
Bone morphogenetic protein 2
03 medical and health sciences
chemistry.chemical_compound
Coated Materials
Biocompatible
Osteogenesis
Gram-Negative Bacteria
Cell Adhesion
Humans
Bifunctional
Nanoscopic scale
Cell Proliferation
Titanium
technology
industry
and agriculture
Titanium alloy
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
respiratory system
equipment and supplies
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
stomatognathic diseases
030104 developmental biology
chemistry
Chemical engineering
Hydroxyapatite coating
Hydroxyapatites
0210 nano-technology
Biotechnology
Zdroj: Artificial cells, nanomedicine, and biotechnology. 46(sup3)
ISSN: 2169-141X
Popis: To resolve the problems of bacterial infections and the low efficiency of osteogenesis of implanted titanium alloys in clinical dental and bone therapy, we developed a bifunctional titanium alloy (Ti) with a nano-hydroxyapatite (HA) coating (HBD + BMP/HA-Ti), which enables the sustained release of the natural antimicrobial peptide human β-defensin 3 (HBD-3) and bone morphogenetic protein-2 (BMP-2). Due to the poriferous nano-sized structure of the HA coating with a 20-30 μm thickness, the HBD + BMP/HA-Ti material had a high encapsulation efficiency (74%) and exhibited synchronized slow release of HBD-3 and BMP-2. In an antibacterial test, HBD + BMP/HA-Ti prevented the growth of bacteria in an inoculated medium, and its surface remained free from viable bacteria after a continuous incubation with Gram-negative and Gram-positive strains for 7 days. Furthermore, good adhesion, proliferation and osteogenic differentiation of hBMSCs in contact with HBD + BMP/HA-Ti were achieved in 7 days. Therefore, the bifunctional titanium alloy HBD + BMP/HA-Ti has a great potential for eventual applications in the protection of implants against bacteria in the orthopaedic and dental clinic.
Databáze: OpenAIRE
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