Effect of neurokinins on canine prostate cell physiology
| Autor: | Paul D. Walden, Anthony P.D.W. Ford, Elda Tzoumaka, Anindya Bhattacharya, Harley T. Syyong, Dorene Marinese, Dinesh Srinivasan |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Stromal cell Urology Cellular differentiation Calcitonin Gene-Related Peptide Neurokinin A Immunocytochemistry Substance P Calcitonin gene-related peptide Biology Tritium chemistry.chemical_compound Dogs Prostate Internal medicine medicine Animals Receptor Cells Cultured Receptors Tachykinin Cell Differentiation Epithelial Cells Muscle Smooth Receptors Neurokinin-3 Receptors Neurokinin-2 Fibroblasts Receptors Neurokinin-1 Molecular biology Immunohistochemistry Endocrinology medicine.anatomical_structure Oncology chemistry Quinolines Stromal Cells Cell Division |
| Zdroj: | The Prostate. 63(4) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND Sensory peptide neurotransmitters have been implicated as significant regulators of prostate growth. This study was designed to evaluate the role of neurokinins in proliferation, differentiation, and contraction of canine prostate cells in culture. METHODS NK1, NK2, and NK3 receptor subtypes were localized in canine prostate tissue by immunocytochemistry and ligand binding studies. Functional effects of neurokinin agonists were tested on cell differentiation (expression of smooth muscle actin (SMA)), proliferation (MTS assay), and contraction of canine prostate cells in culture. RESULTS Immunocytochemical staining of canine prostate sections revealed strong stromal staining for NK1 together with weak stromal staining for NK2 and even weaker staining for NK3. Furthermore, there was overlapping localization of NK1 receptors, substance P (SP), and calcitonin gene-regulated peptide (CGRP) in prostate tissue sections. SP caused concentration-dependent increase in SMA expression that was attenuated in a concentration-dependent manner by YM-44778, a non-selective antagonist for neurokinin receptors, but not by either the NK2 antagonist (SR-48968) nor by the NK3 antagonist (SB-223412). SP and neurokinin A (NKA) also caused a modest contraction of stromal cells in collagen gels. NKA stimulated proliferation of prostate epithelial cells without any apoptotic effect, which was attenuated by SR-48968. Surprisingly, in binding studies NK3 appeared to be the most abundant neurokinin receptor subtype, although functional studies failed to reveal significant coupling of this receptor. CONCLUSIONS Our results suggest that, at least in vitro, neurokinins have modest effects on canine prostate epithelial cell proliferation, stromal differentiation, and contraction. © 2004 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text