A Comparison of Substrates for Human Umbilical Vein Endothelial Cell Culture
| Autor: | J. F. M. Leeuwenberg, E. J. U. Von Asmuth, C. J. Van Der Linden, E. F. Smeets, Wim A. Buurman |
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| Rok vydání: | 1992 |
| Předmět: |
Umbilical Veins
Histology food.ingredient Antigens Differentiation Myelomonocytic Gelatin chemistry.chemical_compound food Humans Cells Cultured Staining and Labeling biology Cell adhesion molecule General Medicine Platelet Endothelial Cell Adhesion Molecule-1 Endothelial stem cell Fibronectin Medical Laboratory Technology Silver nitrate Intercellular Junctions chemistry Biochemistry Cell culture embryonic structures cardiovascular system biology.protein Biophysics Silver Nitrate Human umbilical vein endothelial cell Endothelium Vascular Glass Glutaraldehyde Cell Adhesion Molecules Plastics |
| Zdroj: | Biotechnic & Histochemistry. 67:241-250 |
| ISSN: | 1473-7760 1052-0295 |
| Popis: | We have studied culture conditions which facilitate the growth of stable, non-proliferating, human umbilical vein endothelial cell (HUVEC) monolayers. Gelatin and fibronectin coatings, with or without glutaraldehyde cross-linking, on both plastic and glass were investigated for initial attachment of HUVEC and growth characteristics. The presence during culture of intercellular (IC) junctions demonstrated by silver staining, expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) and maintenance of a cobblestone appearance of HUVEC monolayers were assessed over time. Glutaraldehyde cross-linked fibronectin and gelatin coatings on glass and glutaraldehyde cross-linked gelatin or untreated fibronectin coatings on plastic served as good substrates for short term culture. Long term (20 days) cultures of HUVEC which maintained silver and PECAM-1 staining of IC junctions and a cobblestone appearance could be achieved if glutaraldehyde cross-linked gelatin coatings on glass were used as substrates. |
| Databáze: | OpenAIRE |
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