Modulation of protein aggregation by polyethylene glycol conjugation: GCSF as a case study
| Autor: | Tiansheng Li, Robert A. Briddell, Olaf B Kinstler, Michael J. Treuheit, Rahul S. Rajan, David N. Brems, Heather Yeghnazar, Alexis Lueras, Yu Zhang, Hiromi Arai, Christopher James Sloey, Mohini Aras, Weston Sutherland |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chemistry
technology industry and agriculture Proteins Biological activity Polyethylene glycol Protein aggregation Biochemistry Protein Structure Secondary Article Polyethylene Glycols chemistry.chemical_compound Kinetics Structure-Activity Relationship Protein structure Solubility Covalent bond PEG ratio Granulocyte Colony-Stimulating Factor Biophysics Protein precipitation Chemical Precipitation Molecular Biology Copper |
| Zdroj: | Protein science : a publication of the Protein Society. 15(5) |
| ISSN: | 0961-8368 |
| Popis: | Polyethylene glycol (PEG) conjugation to proteins has emerged as an important technology to produce drug molecules with sustained duration in the body. However, the implications of PEG conjugation to protein aggregation have not been well understood. In this study, conducted under physiological pH and temperature, N-terminal attachment of a 20 kDa PEG moiety to GCSF had the ability to (1) prevent protein precipitation by rendering the aggregates soluble, and (2) slow the rate of aggregation relative to GCSF. Our data suggest that PEG-GCSF solubility was mediated by favorable solvation of water molecules around the PEG group. PEG-GCSF appeared to aggregate on the same pathway as that of GCSF, as evidenced by (a) almost identical secondary structural transitions accompanying aggregation, (b) almost identical covalent character in the aggregates, and (c) the ability of PEG-GCSF to rescue GCSF precipitation. To understand the role of PEG length, the aggregation properties of free GCSF were compared to 5kPEG-GCSF and 20kPEG-GCSF. It was observed that even 5kPEG-GCSF avoided precipitation by forming soluble aggregates, and the stability toward aggregation was vastly improved compared to GCSF, but only marginally less stable than the 20kPEG-GCSF. Biological activity measurements demonstrated that both 5kPEG-GCSF and 20kPEG-GCSF retained greater activity after incubation at physiological conditions than free GCSF, consistent with the stability measurements. The data is most compatible with a model where PEG conjugation preserves the mechanism underlying protein aggregation in GCSF, steric hindrance by PEG influences aggregation rate, while aqueous solubility is mediated by polar PEG groups on the aggregate surface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text