Novosphingobium humi sp. nov., isolated from soil of a military shooting range
| Autor: | EunMi Choi, Che Ok Jeon, Sung Kuk Lee, Kyungchul Kim, Jong Woo Hyeon, Ah Ryeong Son |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial Firearms Novosphingobium Sequence analysis Spermidine Ubiquinone medicine.disease_cause Microbiology 03 medical and health sciences Phylogenetics RNA Ribosomal 16S Republic of Korea medicine Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology Base Composition Phylogenetic tree biology Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Sphingomonadaceae 030104 developmental biology Biochemistry Novosphingobium sediminicola lipids (amino acids peptides and proteins) Soil microbiology |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(8) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-stain-negative, strictly aerobic bacterium, designated R1-4T, was isolated from soil from a military shooting range in the Republic of Korea. Cells were non-motile short rods, oxidase-positive and catalase-negative. Growth of R1-4T was observed at 15–45 °C (optimum, 30 °C) and pH 6.0–9.0 (optimum, pH 7.0). R1-4T contained summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), cyclo-C19 : 0ω8c and C16 : 0 as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, an unknown glycolipid and four unknown lipids were detected as polar lipids. The major polyamine was spermidine. The G+C content of the genomic DNA was 64.4 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that R1-4T formed a tight phylogenetic lineage with Novosphingobium sediminicola HU1-AH51T within the genus Novosphingobium . R1-4T was most closely related to N. sediminicola HU1-AH51T with a 98.8 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness between R1-4T and the type strain of N. sediminicola was 37.8±4.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that R1-4T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium humi sp. nov. is proposed. The type strain is R1-4T (=KACC 19094T=JCM 31879T). |
| Databáze: | OpenAIRE |
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