Effects of Subchronic Inhalation Exposure to Ethyl Tertiary Butyl Ether on Splenocytes in Mice
| Autor: | Toshihiro Kawamoto, Megumi Suda, Kimiko Hirata, Tomoyuki Kawada, Maiko Kobayashi, Takako Shimizu, Qing Li, Tamie Nakajima, Wang Rs, Yukiyo Hirata, Hirofumi Inagaki |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Mice 129 Strain Time Factors T-Lymphocytes CD3 T cell Immunology Spleen Immunophenotyping Flow cytometry Mice Antigens CD Internal medicine medicine Splenocyte Animals Immunology and Allergy Lymphocyte Count Pharmacology Inhalation exposure Air Pollutants B-Lymphocytes Inhalation Exposure Dose-Response Relationship Drug Inhalation biology medicine.diagnostic_test Chemistry Macrophages Body Weight Organ Size Flow Cytometry Killer Cells Natural Mice Inbred C57BL Ethyl Ethers medicine.anatomical_structure Endocrinology Anesthesia biology.protein CD8 circulatory and respiratory physiology |
| Zdroj: | International Journal of Immunopathology and Pharmacology. 24:837-847 |
| ISSN: | 2058-7384 |
| Popis: | Ethyl tertiary-butyl ether (ETBE) is a motor fuel oxygenate used in reformulated gasoline. The current use of ETBE in gasoline or petrol is modest but increasing. To investigate the effects of ETBE on splenocytes, mice were exposed to 0 (control), 500 ppm, 1750 ppm, or 5000 ppm of ETBE by inhalation for 6 h/day for 5 days/wk over a 6- or 13-week period. Splenocytes were harvested from the control and exposed mice, and the following cell phenotypes were quantified by flow cytometry: (1) B cells (PerCP-Cy5.5-CD45R/B220), (2) T cells (PerCP-Cy5-CD3e), (3) T cell subsets (FITC-CD4 and PE-CD8a), (4) natural killer (NK) cells (PE-NK1.1), and (5) macrophages (FITC-CD11b). Body weight and the weight of the spleen were also examined. ETBE-exposure did not affect the weight of the spleen or body weight, while it transiently increased the number of RBC and the Hb concentration. The numbers of splenic CD3+, CD4+, and CD8+ T cells, the percentage of CD4+ T cells and the CD4+/CD8+ T cell ratio in the ETBE-exposed groups were significantly decreased in a dose-dependent manner. However, ETBE exposure did not affect the numbers of splenic NK cells, B cells, or macrophages or the total number of splenocytes. The above findings indicate that ETBE selectively affects the number of splenic T cells in mice. |
| Databáze: | OpenAIRE |
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