A Novel Digestive α-Amylase from Blue Crab (Portunus segnis) Viscera: Purification, Biochemical Characterization and Application for the Improvement of Antioxidant Potential of Oat Flour
| Autor: | Moncef Nasri, Hana Maalej, Amina Maalej, Noomen Hmidet, Sawsan Affes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine purification Starch blue crab food industries 01 natural sciences Catalysis digestive α-amylase Inorganic Chemistry Sepharose lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Hydrolysis 010608 biotechnology Maltotriose Amylase Food science Physical and Theoretical Chemistry Molecular Biology Potato starch lcsh:QH301-705.5 Spectroscopy Portunus segnis biology Chemistry biochemical characterization Organic Chemistry food and beverages General Medicine Maltose Computer Science Applications oat flour 030104 developmental biology antioxidant potential lcsh:Biology (General) lcsh:QD1-999 Sephadex biology.protein |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 1070, p 1070 (2021) International Journal of Molecular Sciences Volume 22 Issue 3 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | This study reports on the purification and characterization of a digestive &alpha amylase from blue crab (Portunussegnis) viscera designated Blue Crab Amylase (BCA). The enzyme was purified to homogeneity by ultrafiltration, Sephadex G-100 gel filtration and Sepharose mono Q anion exchange chromatography, with the final purification fold of 424.02, specific activity of 1390.8 U mg&minus 1 and 27.8% recovery. BCA, showing a molecular weight of approximately 45 kDa, possesses desirable biotechnological features, such as optimal temperature of 50 ° C, interesting thermal stability which is enhanced in the presence of starch, high stability towards surfactants (Tween 20, Tween 80 and Triton X-100), high specific activity, quite high storage and broad pH range stability. The enzyme displayed Km and Vmax values, of 7.5 ± 0.25 mg mL&minus 1 and 2000 ± 23 &mu mol min&minus 1 mg&minus 1 for potato starch, respectively. It hydrolyzed various carbohydrates and produced maltose, maltotriose and maltotetraose as the major end products of starch hydrolysis. In addition, the purified enzyme was successfully utilized for the improvement of the antioxidant potential of oat flour, which could be extended to other cereals. Interestingly, besides its suitability for application in different industrial sectors, especially food industries, the biochemical properties of BCA from the blue crab viscera provide novel features with other marine-derived enzymes and better understanding of the biodegradability of carbohydrates in marine environments, particularly in invasive alien crustaceans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|