Correction of the NSE concentration in hemolyzed serum samples improves its diagnostic accuracy in small-cell lung cancer
| Autor: | Ben van den Borne, Maarten A. C. Broeren, Esther Visser, Huub Belderbos, Maggy Youssef El Soud, Joost L. J. van Dongen, Federica Eduati, Luc Brunsveld, Gerben Stege, Marleen E A de Saegher, Sylvia Genet, Volkher Scharnhorst, Daan van de Kerkhof |
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| Přispěvatelé: | Chemical Biology, Computational Biology, Bio-Organic Chemistry, ICMS Core, EAISI Health |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Enolase Diagnostic accuracy SDG 3 – Goede gezondheid en welzijn Gastroenterology Small-cell lung cancer 03 medical and health sciences 0302 clinical medicine Hemolysis correction equation SDG 3 - Good Health and Well-being Internal medicine Medicine In patient Lung cancer business.industry Protein tumor markers medicine.disease Serum samples Hemolysis 030104 developmental biology Oncology 030220 oncology & carcinogenesis Biomarker (medicine) Non small cell business Neuron-specific enolase Research Paper |
| Zdroj: | Oncotarget, 11(27), 2660-2668. Impact Journals Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Neuron-specific enolase (NSE) is a well-known biomarker for the diagnosis,prognosis and treatment monitoring of small-cell lung cancer (SCLC). Nevertheless, its clinical applicability is limited since serum NSE levels are influenced by hemolysis, leading to falsely elevated results. Therefore, this study aimed to develop a hemolysis correction equation and evaluate its role in SCLC diagnostics.Two serum pools were spiked with increasing amounts of hemolysate obtainedfrom multiple individuals. A hemolysis correction equation was obtained by analyzing the relationship between the measured NSE concentration and the degree of hemolysis. The equation was validated using intentionally hemolyzed serum samples, which showed that the correction was accurate for samples with an H-index up to 30 μmol/L. Correction of the measured NSE concentration in patients suspected of lung cancer caused an increase in AUC and a significantly lower cut-off value for SCLC detection when compared to uncorrected results.Therefore, a hemolysis correction equation should be used to correct falselyelevated NSE concentrations. Results of samples with an H-index above 30 μmol/Lshould not be reported to clinicians. Application of the equation illustrates theimportance of hemolysis correction in SCLC diagnostics and questions the correctness of the currently used diagnostic cut-off value. |
| Databáze: | OpenAIRE |
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