The impact of hepatic xanthine oxidase and xanthine dehydrogenase activities on liver function in chronic cholestasis
| Autor: | Manfred Ratschek, P. Pesendorfer, A.-M. Kuesz, G. Schimpl, Michael E. Höllwarth |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Xanthine Oxidase medicine.medical_specialty Xanthine Dehydrogenase Bilirubin Allopurinol medicine.medical_treatment Tungsten Rats Sprague-Dawley Random Allocation chemistry.chemical_compound Cholestasis Internal medicine medicine Animals Enzyme Inhibitors Xanthine oxidase business.industry Vitamin E General Medicine Glutathione medicine.disease Rats Endocrinology Liver chemistry Xanthine dehydrogenase Chronic Disease Pediatrics Perinatology and Child Health Surgery Liver function business medicine.drug |
| Zdroj: | Pediatric Surgery International. 16:297-301 |
| ISSN: | 1437-9813 0179-0358 |
| Popis: | Activities of hepatic xanthine oxidase (XO) and xanthine dehydrogenase (XD), serum liver enzymes, and reduced glutathione (GSH) were determined in livers of chronic cholestatic rats. The common bile duct was ligated (CBDL) and rats were randomized to either an untreated group or to treatment with allopurinol, a competitive XO inhibitor, or received a tungsten-supplemented diet to inactivate XO and XD, or received antioxidants vitamin C and vitamin E. One group underwent only sham laparotomy. After 4 weeks, in untreated CBDL animals serum aspartate aminotransferase and bilirubin concentrations were significantly elevated and hepatic GSH was significantly decreased when compared with the sham-operated group. Histochemical and enzymatic determinations of XD and XO showed a significant increase in hepatic XO activity after CBDL. Treatment with allopurinol and a tungsten-supplemented, molybdenum-free diet significantly attenuated serum liver enzymes, hepatic XO activity, and improved hepatic GSH levels, whereas vitamins C and E had a positive effect only on hepatic GSH levels. Our results support the hypothesis that cholestasis-induced hepatocellular injury is partially triggered by oxidative processes derived from increased hepatic XO activity. Inhibition and inactivation of XO exerts a hepatocellular protective effect in chronic cholestasis. |
| Databáze: | OpenAIRE |
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