Leukotoxicity of pyoverdin, production of reactive oxygen species, and effect of UV radiation
| Autor: | Inés Albesa, Alberto Jorge Eraso, C. Becerra |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lysis
Cell Survival Neutrophils Ultraviolet Rays Biophysics In Vitro Techniques Endocytosis medicine.disease_cause Pseudomonas fluorescens Biochemistry law.invention Pigment Bacterial Proteins law medicine Humans Molecular Biology Chemiluminescence chemistry.chemical_classification Reactive oxygen species Nitroblue Tetrazolium Cell Biology Metabolism Pigments Biological Darkness Genistein Kinetics chemistry Mechanism of action visual_art Luminescent Measurements visual_art.visual_art_medium medicine.symptom Reactive Oxygen Species Oligopeptides Oxidative stress |
| Zdroj: | Biochemical and biophysical research communications. 285(2) |
| ISSN: | 0006-291X |
| Popis: | Pyoverdin was purified by solvent extraction, gel filtration, and ionic exchange chromatography. Assays of cytotoxic of pyoverdin were done with human leukocytes and macrophages from the peritoneum of mice. Both cell quantities showed a significant reduction. Death was followed by lysis in a dose-dependent form. The mechanism of action of pyoverdin involved the stimulation of reactive oxygen species (ROS) measured by Nitroblue Tetrazolium (NBT) reaction and chemiluminescence (CL). UV radiation at 368 nm increased the leukotoxicity; expositions of 5 min were enough to photostimulate the effect of pyoverdin on cellular oxydative metabolism, which increased between 35.4 and 53.2%. Genestein, an inhibitor of tyrosine kinases, counteracted the ROS stimuli of pyoverdin, suggesting endocytic mechanism of action for this pigment. The little chloroquine interference on oxydative stress indicated that intraphagosomal pH and the stimuli of reactive nitrogen intermediaries (RNI) seem to be of less importance than ROS in pyoverdin action on leukocytes. |
| Databáze: | OpenAIRE |
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