Point mutations in dimerization motifs of the transmembrane domain stabilize active or inactive state of the EphA2 receptor tyrosine kinase
| Autor: | M. V. Astapova, George V. Sharonov, Peter M. Kolosov, Eduard V. Bocharov, Alexey V. Feofanov, Alexander S. Arseniev |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Protein domain Amino Acid Motifs Biochemistry Receptor tyrosine kinase Cell surface receptor Membrane Biology Humans Point Mutation Kinase activity Molecular Biology Binding Sites Microscopy Confocal biology Receptor EphA2 Cell Biology Flow Cytometry Molecular biology biological factors Protein Structure Tertiary Transmembrane domain Heptad repeat stomatognathic diseases Luminescent Proteins HEK293 Cells Membrane protein biology.protein Signal transduction biological phenomena cell phenomena and immunity Protein Multimerization human activities |
| Zdroj: | The Journal of biological chemistry. 289(21) |
| ISSN: | 1083-351X |
| Popis: | The EphA2 receptor tyrosine kinase plays a central role in the regulation of cell adhesion and guidance in many human tissues. The activation of EphA2 occurs after proper dimerization/oligomerization in the plasma membrane, which occurs with the participation of extracellular and cytoplasmic domains. Our study revealed that the isolated transmembrane domain (TMD) of EphA2 embedded into the lipid bicelle dimerized via the heptad repeat motif L(535)X3G(539)X2A(542)X3V(546)X2L(549) rather than through the alternative glycine zipper motif A(536)X3G(540)X3G(544) (typical for TMD dimerization in many proteins). To evaluate the significance of TMD interactions for full-length EphA2, we substituted key residues in the heptad repeat motif (HR variant: G539I, A542I, G553I) or in the glycine zipper motif (GZ variant: G540I, G544I) and expressed YFP-tagged EphA2 (WT, HR, and GZ variants) in HEK293T cells. Confocal microscopy revealed a similar distribution of all EphA2-YFP variants in cells. The expression of EphA2-YFP variants and their kinase activity (phosphorylation of Tyr(588) and/or Tyr(594)) and ephrin-A3 binding were analyzed with flow cytometry on a single cell basis. Activation of any EphA2 variant is found to occur even without ephrin stimulation when the EphA2 content in cells is sufficiently high. Ephrin-A3 binding is not affected in mutant variants. Mutations in the TMD have a significant effect on EphA2 activity. Both ligand-dependent and ligand-independent activities are enhanced for the HR variant and reduced for the GZ variant compared with the WT. These findings allow us to suggest TMD dimerization switching between the heptad repeat and glycine zipper motifs, corresponding to inactive and active receptor states, respectively, as a mechanism underlying EphA2 signal transduction. |
| Databáze: | OpenAIRE |
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