Identification of Beet Western Yellows Luteovirus Genes Implicated in Viral Replication and Particle Morphogenesis
| Autor: | Hubert Guilley, D. Scheidecker, Hervé Lot, Kenneth Richards, A. Reutenauer, Gérard Jonard, Véronique Ziegler-Graff |
|---|---|
| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Rok vydání: | 1993 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences Genes Viral viruses Molecular Sequence Data Luteovirus RNA-dependent RNA polymerase Virus Replication 01 natural sciences CHENOPODE Plant Viruses Open Reading Frames 03 medical and health sciences Virology Morphogenesis Point Mutation ORFS Gene Sequence Deletion 030304 developmental biology Genetics 0303 health sciences Base Sequence biology Beet western yellows virus Point mutation Blotting Northern biology.organism_classification [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy 3. Good health Microscopy Electron Open reading frame Viral replication [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology DNA Viral Mutation 010606 plant biology & botany |
| Zdroj: | Virology Virology, Elsevier, 1993, 195, pp.692-699. ⟨10.1006/viro.1993.1420⟩ |
| ISSN: | 0042-6822 1096-0341 |
| DOI: | 10.1006/viro.1993.1420 |
| Popis: | International audience; The roles in replication and viral assembly of different beet western yellows luteovirus gene products were investigated in Chenopodium quinoa protoplasts using mutated transcripts. Of the six long open reading frames (ORFs) present on the viral RNA, only ORFs 2 and 3, which encode proteins containing conserved putative replicase domains, were essential for replication. Various deletions in the 3′ part of the genome within ORFs 4, 5, and 6 did not affect viral replication. Analysis of the progeny of those mutants capable of replication showed that virus particles were produced in protoplasts infected with transcripts modified in ORFs 1, 5, or 6 but not with transcripts unable to produce coat protein, encoded by ORF 4. |
| Databáze: | OpenAIRE |
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