Development-related Expression of KGF and FGF-10 mRNA in the Canine Prostate Gland
| Autor: | F. Shidaifat |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Fibroblast Growth Factor 7 Stromal cell Vimentin Biology Fibroblast growth factor Pathology and Forensic Medicine chemistry.chemical_compound Dogs Prostate Internal medicine medicine Animals RNA Messenger Receptor Fibroblast Growth Factor Type 2 Receptor Messenger RNA FGF10 General Veterinary Gene Expression Regulation Developmental Molecular biology Endocrinology medicine.anatomical_structure chemistry biology.protein Keratinocyte growth factor Fibroblast Growth Factor 10 |
| Zdroj: | Journal of Veterinary Medicine Series A. 54:549-552 |
| ISSN: | 1439-0442 0931-184X |
| DOI: | 10.1111/j.1439-0442.2007.00983.x |
| Popis: | Summary Keratinocyte growth factor (KGF) and fibroblast growth factor 10 (FGF-10) are stromal-derived growth factors that interact with their epithelial FGFR2 receptors to mediate stromal--epithelial cell interaction within the prostate gland. This study was conducted to compare the development-related mRNA expression of KGF, FGF-10 and their receptor FGFR2 in immature and mature canine prostate glands. In addition, their expression levels were correlated with the differentiation of stromal cells using vimentin as a mesenchymal cell marker. Quantitative mRNA expression was assessed by real-time polymerase chain reaction (PCR) and the results were expressed as relative mRNA expression of the target gene, which was normalized to the GAPDH reference gene. mRNA analysis revealed a differential expression of KGF, FGF-10 and FGFR2 receptor by the prostate glands of immature and mature dogs. The results showed a 7.3- and 9-fold decrease in mRNA expression of KGF and FGF-10 by mature and immature prostate glands respectively. However, there was no significant change in FGFR2 receptor mRNA expression by mature or immature prostate glands. This downregulation of KGF and FGF-10 expression was associated with a 15-fold decrease in vimentin expression. These results indicate that KGF and FGF-10 expression varied according to the differentiation status of stromal cells and might reflect differential developmental requirements of immature and mature canine prostate glands. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|