Confluence-induced alterations in CpG island methylation in cultured normal human fibroblasts
| Autor: | Kathryn A. Lester, Christie P. Fanton, Russell O. Pieper |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
Methyltransferase Population Cell Count Simian virus 40 Biology Cell Line O(6)-Methylguanine-DNA Methyltransferase Genetics Humans Sulfites Epigenetics Promoter Regions Genetic education Cellular Senescence Cell Line Transformed education.field_of_study O-6-methylguanine-DNA methyltransferase DNA Methylation DNA Methylation Fibroblasts Cadherins Molecular biology Receptors Estrogen CpG site DNA methylation CpG Islands Cell aging Cell Division Research Article |
| Zdroj: | Nucleic Acids Research. 27:3229-3235 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/27.15.3229 |
| Popis: | Growth constraint of bacterial and human cells has been shown to trigger genetic mutation. We questioned whether growth constraint might also trigger epigenetic mutation in the form of CpG island methylation. Logarithmically growing normal human fibro-blasts (NHF) displayed little (0-15%) CpG methylation in select regions of three CpG islands [estrogen receptor (ER), E-cadherin (ECAD) and O (6)-methylguanine-DNA methyltransferase (MGMT)] examined. NHF grown to and left at confluence for 2-21 days showed little ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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