Survey of SARS-CoV-2 genetic diversity in two major Brazilian cities using a fast and affordable Sanger sequencing strategy
| Autor: | Karine Lima Lourenço, Hugo Itaru Sato, Renata Barbosa Peixoto, Flávio Guimarães da Fonseca, Alex Fiorini, Ana Paula Salles Moura Fernandes, Luciano M. Thomazelli, Bruna Larotonda Telezynski, Guilherme Pereira Scagion, Erick Gustavo Dorlass, Rubens Daniel Miserani Magalhães, Helena Perez Coelho, Santuza M. R. Teixeira, Edison Luiz Durigon, Danielle Bruna Leal Oliveira, Tatiana Ometto |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Sanger sequencing
China Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike Biology Genome Virus Article Measles virus symbols.namesake Genotype Genetics Humans Cities Phylogeny RNA MENSAGEIRO Sars-Cov-2 Genetic diversity Transmission (medicine) genetic variants COVID-19 Genetic Variation High-Throughput Nucleotide Sequencing biology.organism_classification Mutation Spike Glycoprotein Coronavirus symbols Brazil |
| Zdroj: | Genomics Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1089-8646 0888-7543 |
| Popis: | Genetic variants of SARS-CoV-2 have been emerging and circulating in many places across the world. Rapid detection of these variants is essential since their dissemination can impact transmission rates, diagnostic procedures, disease severity, response to vaccines or patient management. Sanger sequencing has been used as the preferred approach for variant detection among circulating human immunodeficiency and measles virus genotypes. Using primers to amplify a fragment of the SARS-CoV-2 genome encoding part of the Spike protein, we showed that Sanger sequencing allowed us to rapidly detect the introduction and spread of three distinct SARS-CoV-2 variants in two major Brazilian cities. In both cities, after the predominance of variants closely related to the virus first identified in China, the emergence of the P.2 variant was quickly followed by the identification of the P1 variant, which became dominant in less than one month after it was first detected. |
| Databáze: | OpenAIRE |
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