| Autor: |
Tania, Dexter, Oluwatosin, Taiwo, Dima, El-Sharkawi, Claire, Dearden, Sunil, Iyengar |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Clinical Lymphoma Myeloma and Leukemia. 22:S401 |
| ISSN: |
2152-2650 |
| Popis: |
Establishing clonality is an important step towards diagnosing B and T-cell lymphomas. While B-cell clonality is established quickly using flow cytometry, the gold standard for T-cell clonality involves expensive and time-consuming genetic studies. The significance of T-cell clones detected by this method are often more uncertain. More recently, TRBC1 expression on T-cells as detected by flow cytometry has been shown to be a simple, rapid and robust representation of T-cell clonality. This has been implemented in our diagnostic laboratory with clonality reported when TRBC1 expression is identified in15% or85% of T-cells. This study examines the reporting of TRBC1 based clonality using flow cytometry and molecular clonality results in the investigation of T-cell lymphocytosis.All cases tested for T-cell clonality by the molecular laboratory at RMH between 05/04/2021 and 10/11/2021 were identified retrospectively. Molecular and immunophenotyping results were extracted from electronic patient records (EPR). We analyzed TRBC1 status and T-cell receptor (TCR) clonality by molecular rearrangements including TCR beta (TCRB) and TCR gamma (TCRG).284 cases were identified. TRBC1 status as detected by flow cytometry was documented in 82 cases. Our laboratory reported a clonal population by TRBC1 in 27/82 cases. In all 27 cases a clonal T-cell population was also identified in both TCRB and TCRG by molecular testing. In 1 case molecular studies failed due to lack of amplification. No clonal TRBC1 population was described as per laboratory cut-offs in 55 cases. 17/55 were also reported as polyclonal by molecular studies. A weak clonal population of uncertain significance was detected in 4/55 cases. There were clonal populations detected by molecular studies in 31/55 of these cases. In 3/55 cases molecular studies failed.100% of cases with clonal TRBC1 detected by flow cytometry demonstrate T-cell clonality on molecular testing. As per current reporting standards defined in our laboratory, small T cell clones are not reported due to the unknown clinical significance leading to a discrepancy between reports by flow and molecular laboratories. The clinical context and outcomes of these cases is being investigated to understand the clinical significance. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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