Entamoeba histolytica Phagocytosis of Human Erythrocytes Involves PATMK, a Member of the Transmembrane Kinase Family
| Autor: | William A. Petri, Alicia S. Linford, Douglas R. Boettner, Christopher D. Huston, Eric R. Houpt, Sarah N. Buss, Nicholas E. Sherman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Erythrocytes
Cytotoxicity Protozoan Proteins Mice Phagosomes Biology (General) RNA Small Interfering Integral membrane protein Phagosome 0303 health sciences Amebiasis Erythrophagocytosis Transmembrane protein 3. Good health Parasite Dysentery Amebic Antibody Research Article QH301-705.5 Phagocytosis Immunology Molecular Sequence Data Biology Microbiology Host-Parasite Interactions Entamoeba Histolytica 03 medical and health sciences Entamoeba histolytica Virology Genetics Animals Humans Amino Acid Sequence Antibodies Blocking Molecular Biology 030304 developmental biology 030306 microbiology Membrane Proteins Cell Biology RC581-607 biology.organism_classification Disease Models Animal Membrane protein biology.protein Mice Inbred CBA Parasitology Immunologic diseases. Allergy Gerbillinae Protein Kinases |
| Zdroj: | PLoS Pathogens PLoS Pathogens, Vol 4, Iss 1, p e8 (2008) |
| ISSN: | 1553-7374 1553-7366 |
| Popis: | Entamoeba histolytica is the cause of amebic colitis and liver abscess. This parasite induces apoptosis in host cells and utilizes exposed ligands such as phosphatidylserine to ingest the apoptotic corpses and invade deeper into host tissue. The purpose of this work was to identify amebic proteins involved in the recognition and ingestion of dead cells. A member of the transmembrane kinase family, phagosome-associated TMK96 (PATMK), was identified in a proteomic screen for early phagosomal proteins. Anti-peptide affinity-purified antibody produced against PATMK demonstrated that it was a type I integral membrane protein that was expressed on the trophozoite surface, and that co-localized with human erythrocytes at the site of contact. The role of PATMK in erythrophagocytosis in vitro was demonstrated by: (i) incubation of ameba with anti-PATMK antibodies; (ii) PATMK mRNA knock-down using a novel shRNA expression system; and (iii) expression of a carboxy-truncation of PATMK (PATMKΔ932). Expression of the carboxy-truncation of PATMKΔ932 also caused a specific reduction in the ability of E. histolytica to establish infection in the intestinal model of amebiasis, however these amebae retained the ability to cause hepatic abscesses when directly injected in the liver. In conclusion, PATMK was identified as a member of the TMK family that participates in erythrophagocytosis and is uniquely required for intestinal infection. Author Summary There is a highly ordered process by which the parasite Entamoeba histolytica interacts with human cells. Adherence via a parasite lectin is followed in seconds by killing, with only the corpse and not a living cell ingested by the ameba. This process is so central to pathogenesis that clinicians use the presence of ingested erythrocytes to identify E. histolytica and distinguish it from harmless commensal amebae of the gut. We hypothesized that identification of molecules involved in the ingestion of the corpse might provide insight into how amebae cause colitis. We identified a member of the transmembrane kinase family as an early component of the phagosome. Inhibition of this kinase blocked red cell ingestion and prevented amebae from colonizing and invading the gut. There was no impact on dominant-negative parasites to cause liver abscess, suggesting the pathogenesis program differs between anatomic sites. Future studies of the transmembrane kinanse in erythrophagocytosis may provide insight into how amebae colonize and invade the gut, with the ultimate goal of preventing disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|