Hypoxia Sensing and Persistence Genes Are Expressed during the Intragranulomatous Survival of Mycobacterium tuberculosis
| Autor: | Michelle Mueller, Uma S. Gautam, Kaylee M. Gentry, Nirmalya Bandyopadhyay, Taylor W. Foreman, Ashley V. Veatch, Robert L. Hunter, Xavier Alvarez, Denae N. LoBato, Smriti Mehra, Deepak Kaushal, Amy Cavigli, Joel S. Bader, Teresa A. Hudock, Andrew A. Lackner, Nadia A. Golden, Shen-An Hwang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Microarray 030106 microbiology Clinical Biochemistry Caseous necrosis Biology Real-Time Polymerase Chain Reaction Regulon Transcriptome Mycobacterium tuberculosis 03 medical and health sciences Gene expression medicine Animals Tuberculosis Anaerobiosis Molecular Biology Gene Lung Original Research Granuloma Microbial Viability Gene Expression Profiling Reproducibility of Results Cell Biology Gene Expression Regulation Bacterial respiratory system medicine.disease biology.organism_classification bacterial infections and mycoses 030104 developmental biology medicine.anatomical_structure Genes Bacterial Immunology Macaca |
| Zdroj: | American journal of respiratory cell and molecular biology. 56(5) |
| ISSN: | 1535-4989 |
| Popis: | Although it is accepted that the environment within the granuloma profoundly affects Mycobacterium tuberculosis (Mtb) and infection outcome, our ability to understand Mtb gene expression in these niches has been limited. We determined intragranulomatous gene expression in human-like lung lesions derived from nonhuman primates with both active tuberculosis (ATB) and latent TB infection (LTBI). We employed a non–laser-based approach to microdissect individual lung lesions and interrogate the global transcriptome of Mtb within granulomas. Mtb genes expressed in classical granulomas with central, caseous necrosis, as well as within the caseum itself, were identified and compared with other Mtb lesions in animals with ATB (n = 7) or LTBI (n = 7). Results were validated using both an oligonucleotide approach and RT-PCR on macaque samples and by using human TB samples. We detected approximately 2,900 and 1,850 statistically significant genes in ATB and LTBI lesions, respectively (linear models for microarray analysis, Bonferroni corrected, P |
| Databáze: | OpenAIRE |
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