Heat-transfer based characterization of DNA on synthetic sapphire chips

Autor: Ken Haenen, Luc Michiels, Weng Siang Yeap, Hans-Gerd Boyen, W. De Ceuninck, Peter Kaul, B. van Grinsven, Bert Conings, Michael J. Schöning, Y. Eurlings, Patrick Wagner, Marcel Ameloot, Johannes Warmer, Mohammed Sharif Murib, Robert Carleer
Jazyk: angličtina
Rok vydání: 2016
Předmět:
SILICON SURFACES
Thermogravimetric analysis
X-ray photoelectron spectroscopy
DIAMOND THIN-FILMS
Materials science
Analytical chemistry
Infrared spectroscopy
Fourier-transform infrared spectroscopy
02 engineering and technology
010402 general chemistry
01 natural sciences
SEQUENCE
PARAMETERS
chemistry.chemical_compound
MOLECULES
BIOSENSOR
Materials Chemistry
Electrical and Electronic Engineering
Fourier transform infrared spectroscopy
Instrumentation
Single-nucleotide polymorphisms in DNA
Metals and Alloys
MONOLAYERS
021001 nanoscience & nanotechnology
Condensed Matter Physics
0104 chemical sciences
Surfaces
Coatings and Films
Electronic
Optical and Magnetic Materials
Chemical state
Confocal fluorescence microscopy
Synthetic sapphire
Nanocrystalline diamond
chemistry
Triethoxysilane
Sapphire
X-RAY
Heat-transfer method
0210 nano-technology
Biosensor
HYBRIDIZATION
INTERFACES
Zdroj: Sensors and Actuators B-Chemical, 230, 260-271. Elsevier Science
ISSN: 0925-4005
Popis: In this study, we show that synthetic sapphire (A1203), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material. (C) 2016 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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