Identification of the sex-determining locus in grass puffer (Takifugu niphobles) provides evidence for sex-chromosome turnover in a subset of Takifugu species

Autor: Sho Hosoya, Satoshi Tasumi, Shota Tajima, Yuzuru Suzuki, Risa Ieda, Takashi Koyama, Aoi Nozawa, Yuma Aoki, Kiyoshi Kikuchi, Kazufumi Atsumi, Osamu Nakamura, Takashi Kamiya
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
0106 biological sciences
0301 basic medicine
Heredity
lcsh:Medicine
01 natural sciences
lcsh:Science
Phylogeny
Recombination
Genetic
Genetics
Sex Chromosomes
Multidisciplinary
biology
Chromosome Biology
Chromosome Mapping
Genomics
Genetic Mapping
Linkage Analysis
Female
Research Article
Takifugu rubripes
Quantitative Trait Loci
Locus (genetics)
Quantitative trait locus
Research and Analysis Methods
Takifugu
Polymorphism
Single Nucleotide
010603 evolutionary biology
Chromosomes
Molecular Genetics
03 medical and health sciences
Gene mapping
Chromosome 19
Animals
Allele
Molecular Biology Techniques
Linkage Mapping
Molecular Biology
Crosses
Genetic
Fugu
Gene Mapping
lcsh:R
Biology and Life Sciences
Computational Biology
Cell Biology
Sex Determination Processes
Comparative Genomics
biology.organism_classification
030104 developmental biology
Genetic Loci
lcsh:Q
Zdroj: PLoS ONE, Vol 13, Iss 1, p e0190635 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: There is increasing evidence for frequent turnover in sex chromosomes in vertebrates. Yet experimental systems suitable for tracing the detailed process of turnover are rare. In theory, homologous turnover is possible if the new sex-determining locus is established on the existing sex-chromosome. However, there is no empirical evidence for such an event. The genus Takifugu includes fugu (Takifugu rubripes) and its two closely-related species whose sex is most likely determined by a SNP at the Amhr2 locus. In these species, males are heterozygous, with G and C alleles at the SNP site, while females are homozygous for the C allele. To determine if a shift in the sex-determining locus occurred in another member of this genus, we used genetic mapping to characterize the sex-chromosome systems of Takifugu niphobles. We found that the G allele of Amhr2 is absent in T. niphobles. Nevertheless, our initial mapping suggests a linkage between the phenotypic sex and the chromosome 19, which harbors the Amhr2 locus. Subsequent high-resolution analysis using a sex-reversed fish demonstrated that the sex-determining locus maps to the proximal end of chromosome 19, far from the Amhr2 locus. Thus, it is likely that homologous turnover involving these species has occurred. The data also showed that there is a male-specific reduction of recombination around the sex-determining locus. Nevertheless, no evidence for sex-chromosome differentiation was detected: the reduced recombination depended on phenotypic sex rather than genotypic sex; no X- or Y-specific maker was obtained; the YY individual was viable. Furthermore, fine-scale mapping narrowed down the new sex-determining locus to the interval corresponding to approximately 300-kb of sequence in the fugu genome. Thus, T. niphobles is determined to have a young and small sex-determining region that is suitable for studying an early phase of sex-chromosome evolution and the mechanisms underlying turnover of sex chromosome.
Databáze: OpenAIRE
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