Downregulation of histone methyltransferase EHMT2 in CD4+ T-cells may protect HTLV-1-infected individuals against HAM/TSP development
| Autor: | Dimas Tadeu Covas, Fabio Pittella Silva, Camila Schoueri Colaço, Katia Kaori Otaguiri, Osvaldo Massaiti Takayanagui, Martha Silva Estrêla, Rodrigo Haddad, Evandra Strazza Rodrigues, Simone Kashima, Maurício Cristiano Rocha-Júnior, Adriano Reis de Matos |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
viruses virus diseases ANTÍGENOS DE HISTOCOMPATIBILIDADE General Medicine Biology medicine.disease Peripheral blood mononuclear cell Virology EHMT2 03 medical and health sciences Leukemia EHMT1 030104 developmental biology Downregulation and upregulation immune system diseases hemic and lymphatic diseases Histone methyltransferase Tropical spastic paraparesis medicine Epigenetics |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-017-3428-8 |
| Popis: | Approximately 5% of human T-cell leukemia virus type 1 (HTLV-1)-infected individuals will develop one of the HTLV-1-related diseases, such as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) or adult T-cell leukemia. However, the mechanisms responsible for the appearance of symptoms have not been fully clarified. It is believed that viral factors, host genetic and epigenetic mechanisms are implicated in this process. Studies have shown the involvement of histone methyltransferases in retrovirus infection, but no study observed their expression in HTLV-1-infected patients. Among them, euchromatic histone-lysine N-methyltransferase (EHMT)-1 and EHMT-2 were related to retroviral latency in HIV-1 infection. We investigated whether histone methyltransferases EHMT1 and EHMT2 exert any influence on HAM/TSP development by assessing their expression levels in CD4+ T-cells from HTLV-1-infected patients. CD4+ T-cells were immunomagnetically isolated from peripheral blood mononuclear cells of HTLV-1-infected or non-infected individuals and the expression levels of EHMT1 and EHMT2 were determined by RT-qPCR. We observed that EHMT2 was negatively regulated in HTLV-1 asymptomatic carriers compared to non-infected individuals. No difference was observed for EHMT1. These results suggest that EHMT2 downregulation in CD4+ T-cells may be linked to a protection mechanism against the development of HAM/TSP. |
| Databáze: | OpenAIRE |
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