Detection of 5–fluorouracil surface contamination in near real time
Autor: | Jerome P. Smith, D Gayle DeBord, Thomas H Connor, Shirley A. Robertson, Kristine Kurtz, Deborah L. Sammons, John E. Snawder, Jack R Pretty |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Drug Contamination
Direct reading Chemistry Pharmaceutical Antineoplastic Agents 02 engineering and technology Tandem mass spectrometry Article 03 medical and health sciences 0302 clinical medicine 0203 mechanical engineering Computer Systems Tandem Mass Spectrometry Medicine Humans Pharmacology (medical) Workplace Chromatography business.industry Chromatography liquid Reproducibility of Results Contamination 020303 mechanical engineering & transports Oncology Fluorouracil 030220 oncology & carcinogenesis Antineoplastic Drugs business medicine.drug Chromatography Liquid |
Popis: | Objectives Contamination of workplace surfaces by antineoplastic drugs presents an exposure risk for healthcare workers. Traditional instrumental methods to detect contamination such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) are sensitive and accurate but expensive and incapable of producing results in real time. This limits their utility in preventing worker exposure. We are currently developing monitors based on lateral flow immunoassay that can detect drug contamination in near real time. In this report, we describe the laboratory performance of a 5-fluorouracil (5-FU) monitor. Methods The monitor was evaluated by spiking ceramic, vinyl, composite, stainless steel, and glass surfaces of 100 cm2 area with 5-FU masses of 0, 5, 10, 25, 50, and 100 ng. The surface was sampled with a wetted cotton swab, the swab was extracted with buffer, and the resulting solution was applied to a lateral flow monitor. Two ways of evaluating the response of these monitors were used: an electronic method where a lateral flow reader was used for measuring line intensities, and a visual method where the intensity of the test line was visually compared to the control line. Results The 5-FU monitor is capable of detecting 10 ng/100 cm2 (0.1 ng/cm2) using the electronic reader and 25 ng/100 cm2 (0.25 ng/cm2) using the visual comparison method for the surfaces studied. The response of the monitors was compared to LC-MS/MS results for the same samples for validation and there was good correlation of the two methods but some differences in absolute response, especially at higher spiking levels for the surface samples. |
Databáze: | OpenAIRE |
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