Urinary excretion of the uraemic toxin p-cresol in the rat: contribution of glucuronidation to its metabolization
| Autor: | Rita De Smet, Frans M. Belpaire, Gerrit Lesaffer, Marijn Van Hulle, Norbert Lameire, Bruno Van Vlem, Raymond Vanholder, Rita Cornelis |
|---|---|
| Přispěvatelé: | Critical Care |
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Urinary system Metabolite uremic toxins Glucuronidation Urine Mass Spectrometry Excretion Cresols chemistry.chemical_compound Glucuronides p-cresol Internal medicine parasitic diseases medicine Animals Biotransformation Chromatography High Pressure Liquid Toxins Biological Uremia Transplantation Creatinine business.industry glucuronidation medicine.disease Rats Disease Models Animal Endocrinology chemistry kinetics Nephrology Glucuronide business |
| Zdroj: | Nephrology Dialysis Transplantation. 18:1299-1306 |
| ISSN: | 1460-2385 0931-0509 |
| DOI: | 10.1093/ndt/gfg107 |
| Popis: | BACKGROUND Increasing evidence indicates that lipophilic and/or protein-bound substances such as p-cresol are responsible for adverse physiological alterations in uraemic patients. To better understand the evolution of p-cresol disposition in renal failure and dialysis patients, it is necessary to determine its kinetic characteristics and biotransformation pathways. METHODS We studied the biotransformation of p-cresol after intravenous injection of the compound in eight rats with normal renal function. Urine was collected in four 1 h intervals. To evaluate the presence of p-cresol metabolites, beta-glucuronidase was added to urine samples and the isolated unidentified chromatographic peak observed in previous experiments was submitted to tandem mass spectrometry (MS/MS) analysis. RESULTS Administration of p-cresol produced a p-cresol peak and an unknown peak, suggesting biotransformation of the compound. Addition of beta-glucuronidase to urine samples and incubation at 37 degrees C resulted in a marked decrease in the unidentified peak height (P |
| Databáze: | OpenAIRE |
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