Guide to electrospinning denatured whole chain collagen from hoki fish using benign solvents
| Autor: | Deborah Le Corre-Bordes, Kathleen Hofman, Bronwyn Hall |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Protein Denaturation
food.ingredient 02 engineering and technology 010402 general chemistry Fibril 01 natural sciences Biochemistry Gelatin Citric Acid Extracellular matrix food Tissue engineering Structural Biology Animals Surface Tension Molecular Biology Acetic Acid chemistry.chemical_classification Tissue Engineering Chemistry Viscosity Electric Conductivity Fishes General Medicine Polymer 021001 nanoscience & nanotechnology Electrospinning 0104 chemical sciences Solutions Chemical engineering Nanofiber Hydrodynamics Solvents Cattle Collagen 0210 nano-technology Type I collagen |
| Zdroj: | International journal of biological macromolecules. 112 |
| ISSN: | 1879-0003 |
| Popis: | Tissue engineering requires the design and manufacture of biomimetic scaffolds. Collagen-derived nanofibrous scaffolds have been intensively studied because collagen, in the form of fibrils, is one of the main components of the extra cellular matrix (ECM). Several collagen materials have been used in electrospinning studies including mammalian extracted Type I collagen and gelatin formulations. Denatured whole chain collagen (DWCC) can be prepared by heat denaturing acid-soluble collagen extracted from cold-water fish skin. This product provides a consistent source of collagen with a controlled molecular weight profile and intact alpha chains including telopeptides. In this work, we studied DWCC-water-acid systems in order to determine the effect of solution composition on nanofibre morphology. Whereas measurement of the classical physical properties of concentrated solutions failed to predict and only partially explained the electrospinning behavior of collagen derived polymers, hydrodynamic properties provided insight. All the samples are presented in ternary diagrams to map the electrospinnability of the systems. These "electrospinning maps" provide an informative resource to electrospinning collagen-derived product for biomedical or commercial applications and a practical alternative to complicated models developed for synthetic polymers. |
| Databáze: | OpenAIRE |
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