Basic fibroblast growth factor is a testicular germ cell product which may regulate Sertoli cell function
| Autor: | Michael D. Griswold, Kwan Hee Kim, Vincent Blanckaert, Margaret E. Schelling, M. P. Mcguinness, In Suk Han, S. R. Sylvester, Subramaniam Venkateswaran |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
endocrine system Blotting Western Basic fibroblast growth factor Cell Communication Spermatocyte Biology Rats Sprague-Dawley chemistry.chemical_compound Endocrinology FGF9 Spermatocytes medicine Animals RNA Messenger Spermatogenesis Molecular Biology Cells Cultured Chromatography High Pressure Liquid Sertoli Cells Leydig cell urogenital system Transferrin Leydig Cells General Medicine Blotting Northern Sertoli cell Immunohistochemistry Precipitin Tests Receptors Fibroblast Growth Factor Spermatozoa Molecular biology Rats medicine.anatomical_structure chemistry Fibroblast growth factor receptor Fibroblast Growth Factor 2 Germ cell |
| Zdroj: | Molecular Endocrinology. 7:889-897 |
| ISSN: | 1944-9917 0888-8809 |
| DOI: | 10.1210/mend.7.7.8413313 |
| Popis: | Previously, a proteinacious factor secreted by a mixture of rat testicular spermatocytes and round spermatids was shown to stimulate transferrin mRNA and protein levels in Sertoli cells. To identify the germ cell-secreted proteins which affect Sertoli cell functions, concentrated germ cell-conditioned medium was fractionated by reverse-phase HPLC. The fraction which eluted at 35% acetonitrile increased transferrin secretion in Sertoli cell cultures 2.4-fold above the basal level. Both the active fraction and a protein extract from cultured germ cells were positive for basic fibroblast growth factor (bFGF) as determined by Western blot analysis and immunoprecipitation. The apparent molecular sizes of the immunoreactive proteins were 30, 27, and 24 kilodaltons (kDa). By immunohistochemistry, bFGF was shown to be present in pachytene spermatocytes and Leydig cells. The bFGF receptor was also examined by immunohistochemistry and found to be present in Leydig cells, round and elongated spermatids, and Sertoli cells. The presence of receptors was more pronounced in stages I-VIII. Western blot analysis confirmed that the receptors were expressed in isolated round spermatids, elongated spermatids, and Sertoli cells. Two major receptor species with apparent molecular sizes of 120 and 145 kDa were detected in the rat testis. Germ cells contained both of these receptors, but Sertoli cells possessed only the 120-kDa receptor. From these experiments, it is evident that bFGF is a germ cell product which may regulate Sertoli cell function. The expression of bFGF and its receptor may be an important component of germ cell-Sertoli cell and/or germ cell-germ cell communication during spermatogenesis. |
| Databáze: | OpenAIRE |
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