Leptin Contributes to the Development of the Corpus Luteum
| Autor: | Michelle R. Garcia |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Infertility
medicine.medical_specialty endocrine system Leptin Female infertility 0402 animal and dairy science Adipokine 030209 endocrinology & metabolism 04 agricultural and veterinary sciences General Medicine Luteal phase Biology medicine.disease 040201 dairy & animal science Article 03 medical and health sciences 0302 clinical medicine Endocrinology medicine.anatomical_structure Internal medicine medicine Conceptus Corpus luteum hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Celldevelopmental biology. 6(3) |
| ISSN: | 2168-9296 |
| Popis: | Fibroblast growth factor 2 (FGF2), angiopoietin 1 (Ang1), and vascular endothelial growth factor (VEGF) are angiogenic factors implicated in the vascular development of the corpus luteum (CL). Each factor is regulated or influenced by leptin in non-ovarian tissues. Moreover, leptin and its receptor, ObRb, have been identified in luteal tissue throughout the luteal phase. Therefore, leptin is hypothesized to influence luteal vasculature through the regulation of FGF2, Ang1, and VEGF. Multiparous, cycling crossbred female goats (does) were allocated to early (n=12), mid (n=8), and late (n=11) stages of the luteal phase for CL collection. Luteal tissue was harvested and either snap frozen in liquid N2, paraffin embedded, or cultured with leptin (0, 10−12, 10−11, 10−10, 10−9, 10−8 M). Tissue was analyzed for FGF2, Ang1, VEGF, ObRb, and leptin expression. Angiopoietin 1, FGF2, VEGF expression was higher (P≤0.001) in the mid-luteal stage than the early stage. Expression decreased (P≤0.001) during the late luteal stage with the exception of VEGF, which remained elevated. In contrast, leptin and ObRb were lowest (P≤0.003) during the mid-luteal stage compared to the early and late stages. All factors were detected in and/or around vessels in early stage tissue compared to mid and late stages. Leptin stimulated (P≤0.02) Ang1, FGF2, and VEGF expression only in early stage luteal cultures. Collectively, these data provide evidence that leptin may be involved in the luteal angiogenic process during the early stage of CL formation. |
| Databáze: | OpenAIRE |
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