Effect of crop rotation and soil cover on alteration of the soil microflora generated by the culture of transgenic plants producing opines

Autor: H. Mansouri, Philippe Oger, Yves Dessaux
Přispěvatelé: Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2000
Předmět:
Crops
Agricultural
DNA
Bacterial
0106 biological sciences
Lotus
MESH: Plant Roots
MESH: Triticum
Opine
Genetically modified crops
Bacterial growth
Arginine
Plant Roots
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Genetics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Lotus corniculatus
Mannitol
Rosales
MESH: Rosales
Soil Microbiology
Triticum
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
2. Zero hunger
0303 health sciences
Rhizosphere
Bacteria
biology
fungi
MESH: Arginine
food and beverages
15. Life on land
Plants
Genetically Modified
MESH: Crops
Agricultural
biology.organism_classification
MESH: DNA
Bacterial
MESH: Bacteria
MESH: Plants
Genetically Modified
MESH: Soil Microbiology
Agronomy
chemistry
MESH: Mannitol
Nopaline
MESH: Environmental Monitoring
Soil microbiology
Environmental Monitoring
010606 plant biology & botany
Zdroj: Molecular Ecology
Molecular Ecology, Wiley, 2000, 9 (7), pp.881-90. ⟨10.1046/j.1365-294x.2000.00940.x⟩
ISSN: 1365-294X
0962-1083
DOI: 10.1046/j.1365-294x.2000.00940.x
Popis: The culture of transgenic Lotus corniculatus plants producing opines, which are bacterial growth substrates, leads to the selection of rhizospheric bacteria able to utilize these substrates. We have investigated the fate of the opine-utilizing community over time under different experimental conditions following elimination of selective pressure exerted by the transgenic plants. These plants were removed from the soil, which was either left unplanted or replanted with wild-type L. corniculatus or wheat plants. The density of opine-utilizing bacteria in the fallow soils remained essentially unchanged throughout the experiment, regardless of the soil of origin (soil planted with wild-type or transgenic plants). When wild-type Lotus plants were used to replace their transgenic counterparts, only the bacterial populations able to utilize the opines were affected. Long-term changes affecting the opine-utilizing bacterial community on Lotus roots was dependent upon the opine studied. The concentration of nopaline utilizers decreased, upon replacement of the transgenic plants, to a level similar to that of normal plants, while the concentration of mannopine utilizers decreased to levels intermediate between transgenic and normal plants. These data indicate that: (i) the opine-utilizing bacterial populations can be controlled in the rhizosphere via plant-exudate engineering; (ii) the interaction between the engineered plants and their root-associated micro-organisms is transgene specific; and (iii) alterations induced by the cultivation of transgenic plants may sometimes be persistent. Furthermore, opine-utilizing bacterial populations can be controlled by crop rotation. Therefore, favouring the growth of a rhizobacterium of agronomic interest via an opine-based strategy appears feasible.
Databáze: OpenAIRE
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