Degradation of 14C-labeled streptococcal cell walls by egg white lysozyme and lysosomal enzymes
| Autor: | Sara E. Miller, R W Wheat, Harry A. Gallis |
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| Rok vydání: | 1976 |
| Předmět: |
Time Factors
Streptococcus pyogenes Immunology In Vitro Techniques Biology medicine.disease_cause Microbiology Cell wall chemistry.chemical_compound Egg White Cell Wall In vivo medicine Humans Carbon Radioisotopes Muramidase chemistry.chemical_classification Carbohydrate Infectious Diseases Enzyme Biochemistry chemistry Parasitology Lysozyme Lysosomes Dialysis Filtration Research Article Egg white |
| Zdroj: | Infection and Immunity. 13:1459-1466 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.13.5.1459-1466.1976 |
| Popis: | The resistance of native and trypsin-treated [14C] glucose-labeled cell walls to degradation by lysozyme and human lysosomal enzymes was confirmed. In contrast, chemically N-acetylated cell walls undergo significant degradation by these enzymes in the pH range of 4.5 to 5.5 without prior removal of the group-specific carbohydrate. N-acetylation after removal of the group A carbohydrate by formamide extraction renders the cell walls considerably more susceptible to these enzymes than by formamaide extraction alone. It appears, therefore, that unless N-acetylation can occur in vivo, streptococcal cell walls are minimally degraded, if at all, by human peripheral blood leukocytes or lysozyme. Examination of leukocyte extracts from normal subjects and patients with post-streptococcal syndromes revealed no qualitative differences in ability to dissolve streptococcal cell walls. |
| Databáze: | OpenAIRE |
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